Question 1

A rare disease is now understood to be caused by a single mutant gene.When the researcher learns that the gene encodes a ________,he says "Oh good! We may be able to treat the disease with recombinant protein produced in E.coli and delivered by injection."

Accepted Answer

A)  protein hormone
B)  cell-surface receptor
C)  ribozyme
D)  component of chromatin
E)  mitochondrial enzymeF) B) and E)
G) A) and E)

Question 2

Which type of disease is LEAST likely to be able to be treated using genome editing with TALEN or CRISPR?

Accepted Answer

A)  Trisomy-21
B)  Leukemia
C)  Cystic fibrosis
D)  HuntingtonsE) C) and D)
F) None of the above

Question 3

In some ciliate protozoa,the codons UAA and UAG encode glutamate,rather than acting as STOP codons.How does this compare to other species?

Accepted Answer

A)  This is unusual - almost all species share the same "universal" genetic code.
B)  This is typical - every species has its own unique genetic code.
C)  This is not unusual - while most species have a similar genetic code,there are often variations for some of the amino acids.D) None of the above
E) A) and C)

Question 4

A fourth type of restriction enzyme is identified that cleaves at precise locations like a type II enzyme,but it also occasionally makes mistakes and cuts elsewhere.Would this type of enzyme be a useful enzyme to use in molecular cloning?

Accepted Answer

A)  Yes,because it cuts correctly sometimes.
B)  No,because it is a precise enzyme.
C)  No,because cutting mistakes could lead to incorrect cloning.
D)  Yes,because cutting mistakes don't matter in molecular cloning.E) B) and C)
F) C) and D)

Question 5

A gel electrophoresis is performed,but when the gel imager is used,no bands appear.The researcher might have forgotten to

Accepted Answer

A)  add electric current.
B)  add antibody.
C)  ligate the DNA fragments together.
D)  include DNA dye.
E)  include an RNA probe.F) D) and E)
G) A) and D)

Question 6

Three scientists are using different forms of PCR for their research.Abby wants to determine changes in gene expression levels in a tissue,so she uses ________.Bob is trying to extract genetic information from a wooly mammoth fossil,so he uses ________.Chris needs to express a gene in bacteria that matches the form expressed in kidney cells,so she uses ________.

Accepted Answer

A)  quantitative RT-PCR; PCR; RT-PCR
B)  PCR; quantitative RT-PCR; RT-PCR
C)  RT-PCR; PCR; quantitative RT-PCR
D)  RT-PCR; quantitative RT-PCR; PCR
E)  quantitative RT-PCR; RT-PCR; PCRF) All of the above
G) A) and D)

Question 7

Identify a sequence that is most likely to be recognized by a restriction enzyme.

Accepted Answer

A)  CGATGC
B)  CCCTTT
C)  TGGCCA
D)  TCATCAE) A) and B)
F) A) and C)

Question 8

An enzyme that has been isolated from retroviruses and is used to generate cDNA is

Accepted Answer

A)  transverse ligase.
B)  reverse transcriptase.
C)  reverse endonuclease.
D)  recombinant RNase.
E)  RNA polymerase.F) B) and D)
G) D) and E)

Question 9

Two strands of DNA that have been cut by an endonuclease can be sealed together by a(n)

Accepted Answer

A)  polymerase enzyme.
B)  ligase enzyme.
C)  exonuclease enzyme.
D)  protease enzyme.
E)  methylase enzyme.F) C) and D)
G) None of the above

Question 10

Organisms containing DNA from an outside source are called

Accepted Answer

A)  transcribed organisms.
B)  compound organisms.
C)  hybrid organisms.
D)  plasmid organisms.
E)  transgenic organisms.F) D) and E)
G) A) and B)

Question 11

Gel ________ is a process that separates DNA or protein fragments according to their size,by causing them to migrate within a gel in response to an electric field.

Accepted Answer

A)  duplication
B)  blotting
C)  electrophoresis
D)  migrationE) B) and D)
F) All of the above

Question 12

Which genes were incorporated into the genome of Golden Rice? (check all that apply)

Accepted Answer

A)  Daffodil phytoene synthase gene
B)  Bacterial carotene desaturase gene
C)  Bacterial beta-galactosidase
D)  Daffodil lycopene beta-cyclase geneE) C) and D)
F) None of the above

Question 13

A researcher wants to create a mouse model for a disease,to allow her team to study possible therapies.The disease is known to be caused by a small deletion that removes a certain gene.Which type of mouse would be the best choice for this purpose?

Accepted Answer

A)  Knockout mouse
B)  Knockin mouse
C)  Conditional knockout mouse activated by a drug
D)  Mutant derived by traditional forward genetics screen
E)  Tissue-specific gene knockoutF) A) and B)
G) B) and D)

Question 14

An insecticidal protein has been discovered in a bacterium known as

Accepted Answer

A)  Escherichia coli.
B)  Agrobacterium tumefaciens.
C)  Bacillus thuringiensis.
D)  Aspergillus sp.E) A) and D)
F) None of the above

Question 15

You have discovered a very small amount of DNA from an ancient organism that you want to save and study.What is the very first thing you should do to allow you to study this DNA in the lab?

Accepted Answer

A)  RT-PCR
B)  Gel electrophoresis
C)  Polymerase chain reaction (PCR) 
D)  Insert the DNA into a vectorE) All of the above
F) A) and C)

Question 16

Enzymes that cleave DNA at specific sites are called ________.

Accepted Answer

A)  vectors
B)  peptidases
C)  restriction endonucleases
D)  DNAsesE) A) and D)
F) All of the above

Question 17

Which of the following two enzymes are needed to construct a cDNA library?

Accepted Answer

A)  Reverse transcriptase and DNA ligase
B)  Reverse transcriptase and Taq polymerase
C)  DNA ligase and Taq polymerase
D)  Taq polymerase and RNA polymeraseE) C) and D)
F) A) and C)

Question 18

A farmer is growing glyphosate-resistant transgenic soybeans.What are you unlikely to see in those fields during the growing season?

Accepted Answer

A)  A tilling machine
B)  Caterpillars
C)  Field mice
D)  IrrigationE) A) and D)
F) None of the above

Question 19

A friend makes the argument that transgenic crops have only been used to enrich large corporations,not to improve people's health.What is the best counterexample?

Accepted Answer

A)  Golden rice
B)  Bt maize
C)  Glyphosate-resistant soybeans
D)  Cre-LoxE) B) and D)
F) A) and B)

Question 20

A technique used to amplify a desired piece of DNA is

Accepted Answer

A)  gel electrophoresis.
B)  RFLP.
C)  PCR.
D)  plasmid replication.
E)  Northern Blot.F) A) and C)
G) B) and E)

Exam 17: Biotechnology

A friend makes the argument that transgenic crops have only been used to enrich large corporations,not to improve people's health.What is the best counterexample?

Correct Answer
verified

A fourth type of restriction enzyme is identified that cleaves at precise locations like a type II enzyme,but it also occasionally makes mistakes and cuts elsewhere.Would this type of enzyme be a useful enzyme to use in molecular cloning?

Correct Answer
verified

A rare disease is now understood to be caused by a single mutant gene.When the researcher learns that the gene encodes a ________,he says "Oh good! We may be able to treat the disease with recombinant protein produced in E.coli and delivered by injection."

Correct Answer
verified

Which of the following two enzymes are needed to construct a cDNA library?

Correct Answer
verified

Organisms containing DNA from an outside source are called

Correct Answer
verified

Correct Answer
verified

A technique used to amplify a desired piece of DNA is

Correct Answer
verified

Which type of disease is LEAST likely to be able to be treated using genome editing with TALEN or CRISPR?

Correct Answer
verified

Two strands of DNA that have been cut by an endonuclease can be sealed together by a(n)

Correct Answer
verified

Which genes were incorporated into the genome of Golden Rice? (check all that apply)

Correct Answer
verified

You have discovered a very small amount of DNA from an ancient organism that you want to save and study.What is the very first thing you should do to allow you to study this DNA in the lab?

Correct Answer
verified

In some ciliate protozoa,the codons UAA and UAG encode glutamate,rather than acting as STOP codons.How does this compare to other species?

Correct Answer
verified

Identify a sequence that is most likely to be recognized by a restriction enzyme.

Correct Answer
verified

A gel electrophoresis is performed,but when the gel imager is used,no bands appear.The researcher might have forgotten to

Correct Answer
verified

An enzyme that has been isolated from retroviruses and is used to generate cDNA is

Correct Answer
verified

Enzymes that cleave DNA at specific sites are called ________.

Correct Answer
verified

A farmer is growing glyphosate-resistant transgenic soybeans.What are you unlikely to see in those fields during the growing season?

Correct Answer
verified

Gel ________ is a process that separates DNA or protein fragments according to their size,by causing them to migrate within a gel in response to an electric field.

Correct Answer
verified

A researcher wants to create a mouse model for a disease,to allow her team to study possible therapies.The disease is known to be caused by a small deletion that removes a certain gene.Which type of mouse would be the best choice for this purpose?

Correct Answer
verified

An insecticidal protein has been discovered in a bacterium known as

Correct Answer
verified

Exam 17: Biotechnology

A friend makes the argument that transgenic crops have only been used to enrich large corporations,not to improve people's health.What is the best counterexample?

Correct AnswerverifiedShow Answer

A fourth type of restriction enzyme is identified that cleaves at precise locations like a type II enzyme,but it also occasionally makes mistakes and cuts elsewhere.Would this type of enzyme be a useful enzyme to use in molecular cloning?

Correct AnswerverifiedShow Answer

A rare disease is now understood to be caused by a single mutant gene.When the researcher learns that the gene encodes a ________,he says "Oh good! We may be able to treat the disease with recombinant protein produced in E.coli and delivered by injection."

Correct AnswerverifiedShow Answer

Which of the following two enzymes are needed to construct a cDNA library?

Correct AnswerverifiedShow Answer

Organisms containing DNA from an outside source are called

Correct AnswerverifiedShow Answer

Correct AnswerverifiedShow Answer

A technique used to amplify a desired piece of DNA is

Correct AnswerverifiedShow Answer

Which type of disease is LEAST likely to be able to be treated using genome editing with TALEN or CRISPR?

Correct AnswerverifiedShow Answer

Two strands of DNA that have been cut by an endonuclease can be sealed together by a(n)

Correct AnswerverifiedShow Answer

Which genes were incorporated into the genome of Golden Rice? (check all that apply)

Correct AnswerverifiedShow Answer

You have discovered a very small amount of DNA from an ancient organism that you want to save and study.What is the very first thing you should do to allow you to study this DNA in the lab?

Correct AnswerverifiedShow Answer

In some ciliate protozoa,the codons UAA and UAG encode glutamate,rather than acting as STOP codons.How does this compare to other species?

Correct AnswerverifiedShow Answer

Identify a sequence that is most likely to be recognized by a restriction enzyme.

Correct AnswerverifiedShow Answer

A gel electrophoresis is performed,but when the gel imager is used,no bands appear.The researcher might have forgotten to

Correct AnswerverifiedShow Answer

An enzyme that has been isolated from retroviruses and is used to generate cDNA is

Correct AnswerverifiedShow Answer

Enzymes that cleave DNA at specific sites are called ________.

Correct AnswerverifiedShow Answer

A farmer is growing glyphosate-resistant transgenic soybeans.What are you unlikely to see in those fields during the growing season?

Correct AnswerverifiedShow Answer

Gel ________ is a process that separates DNA or protein fragments according to their size,by causing them to migrate within a gel in response to an electric field.

Correct AnswerverifiedShow Answer

A researcher wants to create a mouse model for a disease,to allow her team to study possible therapies.The disease is known to be caused by a small deletion that removes a certain gene.Which type of mouse would be the best choice for this purpose?

Correct AnswerverifiedShow Answer

An insecticidal protein has been discovered in a bacterium known as

Correct AnswerverifiedShow Answer

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verified

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Correct Answer
verified

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verified

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Correct Answer
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