Question 1

If a chemical inhibitor of DNA ligase was added to bacterial cells after DNA replication initiation, the outcome would be

Accepted Answer

A)  elongation of both strands would not occur without ligase to join new nucleotides together.
B)  elongation would occur, but Okazaki fragments would not be joined together.
C)  elongation of the lagging strand, but not the leading strand would occur.
D)  normal replication would proceed as DNA ligase is not required for this process.
E)  too many supercoils would build up in the DNA, resulting in a halting of elongation.F) A) and D)
G) B) and E)

Question 2

A virus that infects bacterial cells is known as a

Accepted Answer

A)  retrovirus
B)  reovirus
C)  bacteriophage
D)  bacteriovirus
E)  parvovirusF) A) and E)
G) C) and D)

Question 3

The step of DNA replication in which two replication forks moving in opposite directions may meet is called

Accepted Answer

A)  initiation.
B)  elongation.
C)  termination.
D)  translation.
E)  translocation.F) C) and E)
G) D) and E)

Question 4

A single DNA molecule contains two specific target sites separated by an intervening sequence.If site-specific recombination between these sites causes the intervening sequence to be released as a separate circular DNA molecule containing one target site, then the sites were oriented in

Accepted Answer

A)  opposite directions causing excision.
B)  the same direction causing excision.
C)  opposite directions causing integration.
D)  the same direction causing integration.
E)  the same direction causing inversion.F) B) and D)
G) C) and D)

Question 5

Which one of the following is not an element found in DNA?

Accepted Answer

A)  oxygen
B)  sulfur
C)  nitrogen
D)  phosphorous
E)  hydrogenF) A) and E)
G) B) and E)

Question 6

Both Spo11 and Cas9 are enzymes that create double-strand breaks in DNA.

Accepted Answer

A) True 
 B)False

Question 7

How many replication forks depart from an origin of replication?

Accepted Answer

A)  one
B)  two
C)  three
D)  fourE) A) and C)
F) A) and B)

Question 8

The full chemical name of DNA is

Accepted Answer

A)  derived nucleic acid.
B)  dideoxyribonucleic acid.
C)  denatured ribonucleic acid.
D)  deoxyribonucleic acid.E) B) and C)
F) All of the above

Question 9

The ratio of ________ in DNA is 1:1.

Accepted Answer

A)  guanine to adenine
B)  adenine to thymine
C)  cytosine to adenine
D)  uracil to cytosineE) None of the above
F) A) and D)

Question 10

The polarity of DNA synthesis is

Accepted Answer

A)  5'→3'.
B)  3'→5'.
C)  5'→2'.
D)  2'→5'.E) A) and D)
F) C) and D)

Question 11

The step of DNA replication in which the proteins connect the correct sequence of nucleotides into a continuous new strand is called

Accepted Answer

A)  initiation.
B)  elongation.
C)  termination.
D)  translation.
E)  translocation.F) B) and C)
G) A) and B)

Question 12

Recombination occurs during meiotic

Accepted Answer

A)  anaphase
B)  interphase
C)  prophase
D)  metaphaseE) A) and B)
F) None of the above

Question 13

Which of the following is not true of DNA?

Accepted Answer

A)  It is acidic.
B)  It contains deoxyribose.
C)  It is found in cell nuclei.
D)  It contains phosphate.
E)  It contains amino acids.F) A) and D)
G) C) and D)

Question 14

Avery and colleagues purified a white substance as the transforming principle.Treating this substance with enzymes that destroyed RNA, protein, or polysaccharides did not affect its ability to transform cells, but enzyme that degrades DNA destroyed the transforming ability.These results suggested that

Accepted Answer

A)  their transforming principle was not entirely pure.
B)  the transforming principle appeared to be DNA.
C)  the test for transformation was not accurate.
D)  a single gene could transform bacterial cells.
E)  both DNA and proteins are required for transformation.F) D) and E)
G) A) and E)

Question 15

Suppose that in the future, scientists identify bacteria on Mars and these organisms lack a site-specific recombination system.Which components would allow for site-specific recombination to occur in these bacteria?

Accepted Answer

A)  FRT target sites and Flp recombinase
B)  FRT target sites and Cas9 enzyme
C)  an FRT target site and a loxP site
D)  a synthetic homologous chromosomeE) B) and D)
F) None of the above

Question 16

During DNA replication, an incoming nucleotide is enzymatically joined to the preceding nucleotide by a(n) ________ bond.

Accepted Answer

A)  hydrogen
B)  ionic
C)  phosphodiester
D)  electrostaticE) All of the above
F) A) and C)

Question 17

Transformation in bacteria results from the uptake of which molecule?

Accepted Answer

A)  RNA
B)  DNA
C)  lipid
D)  protein
E)  polysaccharideF) B) and C)
G) B) and E)

Question 18

The normal functions of Spo11 and Cas9 enzymes are different because

Accepted Answer

A)  Spo11 produces double-strand breaks during meiosis, whereas Cas9 functions during mitosis.
B)  Spo11 produces site-specific double-strand breaks during meiosis, whereas Cas9 breaks DNA at random genomic locations.
C)  Spo11 produces double-strand breaks in many genomic locations during meiosis, whereas Cas9 is targeted to specific sequences by a CRISPR RNA.
D)  Spo11 produces double-strand breaks at sequences targeted by RNAs expressed during meiosis, whereas Cas9 has the ability to recognize DNA sequences without an RNA.
E)  Spo11 is unique to yeast, whereas Cas9 occurs in most higher eukaryotes.F) D) and E)
G) C) and D)

Question 19

Homologous recombination occurs in a heterozygote in which alleles D and d differ by a single base pair.The D allele has a G at one position, whereas the d allele has a C at the same position.If branch migration causes heteroduplex formation across this position, what is the expected outcome?

Accepted Answer

A)  Both D and d alleles will remain unchanged because G can base pair with C.
B)  Mismatch repair will identify an abnormal G-G base pair and may convert the D allele to a d allele.
C)  Mismatch repair will identify an abnormal C-G base pair and will ensure that the cell has two copies of each allele.
D)  Mismatches will cause the Holliday junction to be unstable and to resolve by the noncrossover pathway.E) A) and D)
F) C) and D)

Question 20

Exposure of which of the following regions of a molecule of DNA permits the reading of DNA sequence without opening the double helix?

Accepted Answer

A)  phosphate groups
B)  phosphodiester bonds
C)  major groove
D)  minor grooveE) B) and C)
F) A) and D)

Exam 6: DNA Structure, Replication, and Recombination

During DNA replication, an incoming nucleotide is enzymatically joined to the preceding nucleotide by a(n) ________ bond.

Correct Answer
verified

Both Spo11 and Cas9 are enzymes that create double-strand breaks in DNA.

Correct Answer
verified

The polarity of DNA synthesis is

Correct Answer
verified

Transformation in bacteria results from the uptake of which molecule?

Correct Answer
verified

Which of the following is not true of DNA?

Correct Answer
verified

A virus that infects bacterial cells is known as a

Correct Answer
verified

How many replication forks depart from an origin of replication?

Correct Answer
verified

Suppose that in the future, scientists identify bacteria on Mars and these organisms lack a site-specific recombination system.Which components would allow for site-specific recombination to occur in these bacteria?

Correct Answer
verified

Homologous recombination occurs in a heterozygote in which alleles D and d differ by a single base pair.The D allele has a G at one position, whereas the d allele has a C at the same position.If branch migration causes heteroduplex formation across this position, what is the expected outcome?

Correct Answer
verified

The step of DNA replication in which the proteins connect the correct sequence of nucleotides into a continuous new strand is called

Correct Answer
verified

The normal functions of Spo11 and Cas9 enzymes are different because

Correct Answer
verified

The ratio of ________ in DNA is 1:1.

Correct Answer
verified

The full chemical name of DNA is

Correct Answer
verified

Recombination occurs during meiotic

Correct Answer
verified

A single DNA molecule contains two specific target sites separated by an intervening sequence.If site-specific recombination between these sites causes the intervening sequence to be released as a separate circular DNA molecule containing one target site, then the sites were oriented in

Correct Answer
verified

If a chemical inhibitor of DNA ligase was added to bacterial cells after DNA replication initiation, the outcome would be

Correct Answer
verified

Which one of the following is not an element found in DNA?

Correct Answer
verified

The step of DNA replication in which two replication forks moving in opposite directions may meet is called

Correct Answer
verified

Exposure of which of the following regions of a molecule of DNA permits the reading of DNA sequence without opening the double helix?

Correct Answer
verified

Correct Answer
verified

Exam 6: DNA Structure, Replication, and Recombination

During DNA replication, an incoming nucleotide is enzymatically joined to the preceding nucleotide by a(n) ________ bond.

Correct AnswerverifiedShow Answer

Both Spo11 and Cas9 are enzymes that create double-strand breaks in DNA.

Correct AnswerverifiedShow Answer

The polarity of DNA synthesis is

Correct AnswerverifiedShow Answer

Transformation in bacteria results from the uptake of which molecule?

Correct AnswerverifiedShow Answer

Which of the following is not true of DNA?

Correct AnswerverifiedShow Answer

A virus that infects bacterial cells is known as a

Correct AnswerverifiedShow Answer

How many replication forks depart from an origin of replication?

Correct AnswerverifiedShow Answer

Suppose that in the future, scientists identify bacteria on Mars and these organisms lack a site-specific recombination system.Which components would allow for site-specific recombination to occur in these bacteria?

Correct AnswerverifiedShow Answer

Homologous recombination occurs in a heterozygote in which alleles D and d differ by a single base pair.The D allele has a G at one position, whereas the d allele has a C at the same position.If branch migration causes heteroduplex formation across this position, what is the expected outcome?

Correct AnswerverifiedShow Answer

The step of DNA replication in which the proteins connect the correct sequence of nucleotides into a continuous new strand is called

Correct AnswerverifiedShow Answer

The normal functions of Spo11 and Cas9 enzymes are different because

Correct AnswerverifiedShow Answer

The ratio of ________ in DNA is 1:1.

Correct AnswerverifiedShow Answer

The full chemical name of DNA is

Correct AnswerverifiedShow Answer

Recombination occurs during meiotic

Correct AnswerverifiedShow Answer

A single DNA molecule contains two specific target sites separated by an intervening sequence.If site-specific recombination between these sites causes the intervening sequence to be released as a separate circular DNA molecule containing one target site, then the sites were oriented in

Correct AnswerverifiedShow Answer

If a chemical inhibitor of DNA ligase was added to bacterial cells after DNA replication initiation, the outcome would be

Correct AnswerverifiedShow Answer

Which one of the following is not an element found in DNA?

Correct AnswerverifiedShow Answer

The step of DNA replication in which two replication forks moving in opposite directions may meet is called

Correct AnswerverifiedShow Answer

Exposure of which of the following regions of a molecule of DNA permits the reading of DNA sequence without opening the double helix?

Correct AnswerverifiedShow Answer

Correct AnswerverifiedShow Answer

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