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Analysis of a water sample by a series of presumptive, confirmatory, and completed tests that provide an estimate of coliform numbers in the water is called the ______.


A) most probable number (MPN)
B) standard plate count
C) membrane filter method
D) coliform count

E) B) and C)
F) A) and D)

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You made your ?rst batch (ever) of wine about 6 weeks ago. You used a new plastic garbage can, covered with plastic wrap over the top, for the process. Now you get a cup of it out of the container only to ?nd no alcohol--just really bad juice. What went wrong?


A) The plastic of the container released chemicals that killed the yeasts.
B) The yeasts were not under anaerobic conditions.
C) You bought the wrong yeast species at the store.
D) Your yeasts mutated into non-alcohol producing organisms.

E) None of the above
F) All of the above

Correct Answer

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Which of the following statements regarding waterborne pathogens is incorrect?


A) Waterborne pathogens include bacteria, viruses, and protozoans.
B) Waterborne pathogens are primarily Campylobacter, Salmonella, Shigella, Vibrio, and Mycobacterium.
C) Encysted protozoans can survive in water for long periods without a human host.
D) Microbes can enter surface water through ordinary exposure to air and soil.

E) A) and B)
F) A) and C)

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Bacteriophages sprayed on cold cuts are effective against ______.


A) Listeria
B) Clostridium
C) S. aureus
D) Mycobacterium
E) coliforms

F) B) and D)
G) C) and D)

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The yeast used in making bread, beer, and wine is ______.


A) Spirulina
B) Leuconostoc mesenteroides
C) Propionibacterium
D) Saccharomyces cerevisiae
E) Streptococcus lactis

F) All of the above
G) C) and E)

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Which of the following is an example of a secondary metabolite?


A) Toxin produced by species of Clostridium
B) Alcohol produced by Saccharomyces during fermentation
C) Lactic acid made during yogurt production.
D) All of these are secondary metabolites.

E) None of the above
F) C) and D)

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Rennin is added to cheese to help preserve it.

A) True
B) False

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You ask the butcher to grind a sirloin roast to be used for hamburger. You family likes hamburgers rare, so you undercook the meat. Unfortunately, within two days they develop the symptoms of gastrointestinal disease: diarrhea, bloating, and vomiting. Which of the following statements best Describes what happened?


A) The meat grinder was contaminated, and bacteria were transferred and distributed throughout the meat.
B) The meat became contaminated by bacteria from other foods in your refrigerator.
C) The meat became contaminated by bacteria on the barbeque grill, coating both sides of the hamburger patties.
D) The animal was sick before slaughter, and the sirloin was contaminated.

E) A) and C)
F) A) and D)

Correct Answer

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What is the best de?nition of biotransformation?


A) Production of alcohol from a substrate
B) Oxidation of sugar to carbon dioxide
C) Production of a toxin from a nontoxic chemical
D) Use of microorganisms to modify substances not normally used for growth

E) C) and D)
F) B) and D)

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Which is not used as a chemical preservative in food?


A) Antibiotics
B) Organic acids
C) Sul?te
D) Ethylene oxide gas
E) Salt

F) A) and B)
G) A) and C)

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The most prominent water-borne pathogens of recent times include all of the following except ______.


A) Salmonella
B) Staphylococcus
C) Norwalk viruses
D) Cryptosporidium

E) A) and D)
F) All of the above

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You want to make wine that will be 20-25% alcohol, but wine rarely gets to that concentration of alcohol. What can you do to accomplish your goal of high-yield alcohol production?


A) Add more sugar to your starting material
B) Add brandy to your wine
C) Let your fermentation go longer
D) Find an alcohol-tolerant yeast strain

E) A) and D)
F) A) and C)

Correct Answer

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What is made by fermenting milk with Streptococcus thermophilus and Lactobacillus bulgaricus?


A) Yogurt
B) Cheese
C) Sour cream
D) Tofu
E) Kefir

F) B) and D)
G) B) and C)

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The starting substrate for vinegar production is ______.


A) water
B) an alcoholic solution
C) a salt solution
D) fruit juice

E) A) and B)
F) A) and D)

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The organism used to make bleu cheese is ______.


A) Penicillium roqueforti
B) Propionibacterium
C) Micrococcus
D) Leuconostoc mesenteroides
E) Streptococcus lactis

F) All of the above
G) A) and D)

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A 100 ml sample of water is ?ltered through a sterile membrane, and the membrane is transferred to a plate of m-Endo agar. Following overnight incubation at 37°C, 57 green metallic colonies appear. The coliform count is _______.


A) 0.57 per 100 ml
B) 57 per 100 ml
C) 5700 per 100 ml
D) too numerous to count

E) C) and D)
F) All of the above

Correct Answer

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The first product produced by genetic manipulation and recombinant technology was ______.


A) insulin
B) penicillin
C) amylase
D) cortisone

E) A) and D)
F) All of the above

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The first step in sewage treatment is ______.


A) chlorination
B) sedimentation
C) aeration and settling
D) filtration

E) B) and C)
F) A) and B)

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Which of the following is/are microorganism/s that can be used as food?


A) Spirulina
B) Methylophilus methylotrophus
C) Algae
D) Fusarium graminearum
E) All of the choices are correct.

F) A) and B)
G) None of the above

Correct Answer

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Which of the following is not a step in the mass production of an organic substrate in a fermentor?


A) Add enzymes to catalyze all required steps in the process.
B) Harvest cells and purify the product.
C) Adjust the environmental conditions for optimal growth of the organism.
D) Remove waste products and dispose responsibly.
E) Inoculate the sterile media in the growth vessel with the microbe of interest.

F) A) and E)
G) A) and B)

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