Question 1

ELISA tests use a ﬂuorescent dye as a label to trace antigen-antibody reactions.

Accepted Answer

A) True 
 B)False

Question 2

Titer is the amount of _____ in serum.

Accepted Answer

A)   antibody
B)   memory cells
C)   WBC
D)   complement
E)   antigenF) C) and E)
G) A) and B)

Question 3

You have a very sore throat with symptoms indicating strep throat. Since you are taking microbiology right now, you decide that you will take a swab from your throat and swab it onto an enrichment medium like a blood agar plate or a brain-heart infusion agar plate. Why is this not a good idea?

Accepted Answer

A)   Due to the fact that you cannot easily see the sampling site, there is a high probability that you will not be able to obtain your own specimen without contaminating the sample with your own normal biota.
B)   You are on a diet now, and you are afraid that the food that you are eating will in some way impact the growth of the bacteria. As a result, you will not get valid results.
C)   You use a mouthwash a couple of times a day, so you think that the antibacterial properties of the mouthwash will inhibit or kill the oral bacteria and results in invalid results.
D)   All of these choices are correct.E) B) and C)
F) C) and D)

Question 4

Biochemical tests include all of the following except ______.

Accepted Answer

A)   tests for the presence of speciﬁc enzymes
B)   antibiotic sensitivity tests
C)   sugar fermentation tests
D)   gas production tests
E)   ribotyping testsF) A) and D)
G) B) and E)

Question 5

Which test is especially good for bacteria that are not readily cultivated in the lab?

Accepted Answer

A)   Gram stain
B)   Phage test
C)   Direct ﬂuorescence antibody (DF
A)   test
D)   Direct antigen test
E)   Dichotomous key analysisF) None of the above
G) A) and E)

Question 6

An infectious organism isolated from a patient has a sequence on its genome TATTGAAGC. This will hybridize to the RNA probe UAUUGAAGC.

Accepted Answer

A) True 
 B)False

Question 7

Staphylococcus aureus can be identiﬁed by mixing the organism with speciﬁc antibody and visually recognizing a clumping reaction between the antigen and the antibody. This is a/an _______ test.

Accepted Answer

A)   agglutination
B)   microarray
C)   biochemical
D)   ELISAE) C) and D)
F) None of the above

Question 8

A patient's vaginal specimen is sent from the walk-in clinic to the microbiology laboratory. There it is cultured on bacteriological media and then placed into an ambient air incubator set at 22% oxygen and 37 degrees Celsius. The patient is strongly suspected of having gonorrhea. She had sexual contact with a partner recently diagnosed with gonorrhea, and she is now displaying the typical symptoms of a vaginal exudate or ?uid. Neisseria gonorrhoeae is a microaerophilic gram-negative coccus-shaped bacterium. Seventy-two hours later, the culture has still not grown Neisseria gonorrhoeae so the lab reports the results back as negative. Interpret this result.

Accepted Answer

A)   The clinical personnel took the sample from an incorrect body area.
B)   The inoculating loop was too hot while streaking the specimen onto agar medium, and the bacteria were all killed.
C)   Neisseria gonorrhoeae cannot be grown in bacteriological media and requires genetic analysis for identi?cation.
D)   The culture was placed into the wrong environment for Neisseria gonorrhoeae.E) C) and D)
F) B) and D)

Question 9

What type of test will detect cell-associated antigens?

Accepted Answer

A)   Precipitation
B)   Speci?city
C)   Cross-reaction
D)   Sensitivity
E)   AgglutinationF) C) and D)
G) A) and D)

Question 10

Specimen collection ______.

Accepted Answer

A)   must be done under sterile conditions
B)   must utilize aseptic techniques
C)   does not require special handling
D)   is always done by a medical professionalE) B) and C)
F) All of the above

Question 11

The property of a test to detect only a certain antibody or antigen and not to react with any others is ______.

Accepted Answer

A)   sensitivity
B)   precipitation
C)   speciﬁcity
D)   agglutination
E)   cross-reactionF) C) and D)
G) A) and E)

Question 12

The tuberculin test is an example of an in vitro serological test.

Accepted Answer

A) True 
 B)False

Question 13

The ELISA is commonly used for diagnosing salmonellosis.

Accepted Answer

A) True 
 B)False

Question 14

Mycobacterium tuberculosis has traditionally been identi?ed in patient samples using biochemical tests, and more recently through DNA probe analysis. In vivo testing can also be completed using the tuberculin skin test. A more recent test that seems to be surpassing the others is the QuantiFERON-TB gold test. Blood samples are mixed with protein antigens of M. tuberculosis. After 16 to 24 hours of incubation, the amount of the cytokine interferon produced by the patient's cells is measured. If the patient is infected with M. tuberculosis, their WBCs will release interferon in
Response to contact with the tuberculosis protein antigens. This test falls under which category of identi?cation tests?

Accepted Answer

A)   Microarray test
B)   DNA ?ngerprinting test
C)   Immunologic test
D)   Genotypic test
E)   Enzymatic testF) None of the above
G) B) and E)

Question 15

Which of these tests is an in vivo test?

Accepted Answer

A)   Tuberculin test
B)   Western Blot
C)   Agglutination test
D)   PCRE) None of the above
F) A) and B)

Question 16

Serological testing can be used to test each of the following except ______.

Accepted Answer

A)   blood serum
B)   saliva
C)   skin
D)   cerebrospinal ﬂuid
E)   urineF) B) and C)
G) A) and E)

Question 17

In precipitation tests, the antigen ______.

Accepted Answer

A)   is a soluble molecule
B)   -antibody complex settles to the bottom of the tube
C)   is a whole cell
D)   is an insoluble moleculeE) A) and C)
F) A) and B)

Question 18

You are the supervisor of a new reference microbiology laboratory, which will receive clinical specimens for identi?cation of bacterial pathogens. When choosing the types of media to order for culture and isolation procedures in your laboratory, what question(s)  do you need to answer?

Accepted Answer

A)   What type of bacteria will you be culturing?
B)   What are the growth factors required by the bacteria that you will be identifying?
C)   What kind of microbiota will the patient specimens contain?
D)   All of the choices are correct.E) A) and D)
F) A) and C)

Question 19

It is necessary to do lab tests to diagnose all diseases.

Accepted Answer

A) True 
 B)False

Question 20

The polymerase chain reaction requires endonuclease to cut samples of DNA.

Accepted Answer

A) True 
 B)False

Exam 17: Diagnosing Infections

Correct Answer
verified

You are the supervisor of a new reference microbiology laboratory, which will receive clinical specimens for identi?cation of bacterial pathogens. When choosing the types of media to order for culture and isolation procedures in your laboratory, what question(s) do you need to answer?

Correct Answer
verified

Biochemical tests include all of the following except ______.

Correct Answer
verified

The ELISA is commonly used for diagnosing salmonellosis.

Correct Answer
verified

What type of test will detect cell-associated antigens?

Correct Answer
verified

Correct Answer
verified

The tuberculin test is an example of an in vitro serological test.

Correct Answer
verified

Serological testing can be used to test each of the following except ______.

Correct Answer
verified

It is necessary to do lab tests to diagnose all diseases.

Correct Answer
verified

Titer is the amount of _____ in serum.

Correct Answer
verified

In precipitation tests, the antigen ______.

Correct Answer
verified

Staphylococcus aureus can be identiﬁed by mixing the organism with speciﬁc antibody and visually recognizing a clumping reaction between the antigen and the antibody. This is a/an _______ test.

Correct Answer
verified

The property of a test to detect only a certain antibody or antigen and not to react with any others is ______.

Correct Answer
verified

An infectious organism isolated from a patient has a sequence on its genome TATTGAAGC. This will hybridize to the RNA probe UAUUGAAGC.

Correct Answer
verified

Which of these tests is an in vivo test?

Correct Answer
verified

ELISA tests use a ﬂuorescent dye as a label to trace antigen-antibody reactions.

Correct Answer
verified

Specimen collection ______.

Correct Answer
verified

Which test is especially good for bacteria that are not readily cultivated in the lab?

Correct Answer
verified

The polymerase chain reaction requires endonuclease to cut samples of DNA.

Correct Answer
verified

You have a very sore throat with symptoms indicating strep throat. Since you are taking microbiology right now, you decide that you will take a swab from your throat and swab it onto an enrichment medium like a blood agar plate or a brain-heart infusion agar plate. Why is this not a good idea?

Correct Answer
verified

Exam 17: Diagnosing Infections

Correct AnswerverifiedShow Answer

You are the supervisor of a new reference microbiology laboratory, which will receive clinical specimens for identi?cation of bacterial pathogens. When choosing the types of media to order for culture and isolation procedures in your laboratory, what question(s) do you need to answer?

Correct AnswerverifiedShow Answer

Biochemical tests include all of the following except ______.

Correct AnswerverifiedShow Answer

The ELISA is commonly used for diagnosing salmonellosis.

Correct AnswerverifiedShow Answer

What type of test will detect cell-associated antigens?

Correct AnswerverifiedShow Answer

Correct AnswerverifiedShow Answer

The tuberculin test is an example of an in vitro serological test.

Correct AnswerverifiedShow Answer

Serological testing can be used to test each of the following except ______.

Correct AnswerverifiedShow Answer

It is necessary to do lab tests to diagnose all diseases.

Correct AnswerverifiedShow Answer

Titer is the amount of _____ in serum.

Correct AnswerverifiedShow Answer

In precipitation tests, the antigen ______.

Correct AnswerverifiedShow Answer

Staphylococcus aureus can be identiﬁed by mixing the organism with speciﬁc antibody and visually recognizing a clumping reaction between the antigen and the antibody. This is a/an _______ test.

Correct AnswerverifiedShow Answer

The property of a test to detect only a certain antibody or antigen and not to react with any others is ______.

Correct AnswerverifiedShow Answer

An infectious organism isolated from a patient has a sequence on its genome TATTGAAGC. This will hybridize to the RNA probe UAUUGAAGC.

Correct AnswerverifiedShow Answer

Which of these tests is an in vivo test?

Correct AnswerverifiedShow Answer

ELISA tests use a ﬂuorescent dye as a label to trace antigen-antibody reactions.

Correct AnswerverifiedShow Answer

Specimen collection ______.

Correct AnswerverifiedShow Answer

Which test is especially good for bacteria that are not readily cultivated in the lab?

Correct AnswerverifiedShow Answer

The polymerase chain reaction requires endonuclease to cut samples of DNA.

Correct AnswerverifiedShow Answer

You have a very sore throat with symptoms indicating strep throat. Since you are taking microbiology right now, you decide that you will take a swab from your throat and swab it onto an enrichment medium like a blood agar plate or a brain-heart infusion agar plate. Why is this not a good idea?

Correct AnswerverifiedShow Answer

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
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Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
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Correct Answer
verified

Correct Answer
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Correct Answer
verified

Correct Answer
verified

Correct Answer
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