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The polymerase chain reaction can be used for identification of ______.


A) proteins
B) growth characteristics of microorganisms
C) microorganisms
D) antibodies in the patient's serum

E) A) and B)
F) All of the above

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Serological testing relies upon ______.


A) the specificity of the Fc region of antibodies
B) the specificity of the variable regions of antibodies
C) a very high viral or bacterial load in the patient
D) a patient who is not immunocompromised

E) None of the above
F) A) and B)

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Which test is the confirming test for people who initially tested antibody-positive in the screening ELISA test?


A) Radioimmunoassay (RIA)
B) Immunelectrophoresis
C) Western blot
D) Quellung test
E) Ouchterlony double diffusion

F) B) and E)
G) A) and B)

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The indirect ELISA test is commonly used to diagnose infections caused by all of the following except ______.


A) rubella virus
B) hepatitis C virus
C) HIV
D) Helicobacter
E) hepatitis A virus

F) A) and B)
G) A) and D)

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The testing method involving the separation of serum proteins by electrophoresis, their subsequent transfer to special filters, and then probing with patient sera is called ______.


A) the Quellung test
B) a radioimmunoassay (RIA)
C) immunelectrophoresis
D) the Western blot
E) Ouchterlony double diffusion

F) A) and B)
G) A) and C)

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Antibiotics are often added to biochemical media to restrict the growth to only those organisms that are being looked for in the specimens.

A) True
B) False

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Which of the following is not a genotypic method of identification?


A) Direct antigen testing
B) DNA analysis with probes
C) rRNA sequencing
D) Pulse-field gel electrophoresis
E) G + C composition

F) All of the above
G) A) and E)

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When antibodies or other substances in serum cross-react with the test reagents, a false positive result can occur.

A) True
B) False

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Rickettsial infections can be diagnosed by the ______.


A) latex agglutination test
B) antistreptolysin O test
C) Weil-Felix agglutination test
D) rapid plasma reagin test

E) C) and D)
F) All of the above

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The primary advantage of genotypic analysis for identification is that ______.


A) it is easier than any other method
B) it is less expensive than other methods
C) culturing of the organism is not required
D) it is widely available

E) B) and C)
F) A) and D)

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A urine specimen obtained from a patient went to the microbiology lab for a bacterial culture and antibiotic sensitivity testing. The specimen sat out on the processing counter for a couple of hours before being cultured. Twenty fours hours later, the lab report goes into the computer---over 100,000 bacterial cells per milliliter of urine. Which statement is an accurate assessment of these Results?


A) Urine samples have to be taken from the kidney in order to get a true bacterial count.
B) Not all bacteria will grow on bacteriological media, so the total count is grossly underestimated.
C) Urine is a sterile ?uid as it leaves the body, so it is likely that the specimen was mishandled by the nursing staff when the sample was obtained.
D) The bacterial count is not accurate since the culture sat without refrigeration.

E) All of the above
F) B) and D)

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Depending on the organism, direct examination of a specimen is often the most rapid diagnostic tool used in the clinical microbiology lab. You have a sample of pus from a patient's penis. What will be the easiest test to con?rm that the patient has gonorrhea?


A) Microarray
B) ELISA
C) Agglutination reaction
D) Gram stain

E) B) and D)
F) A) and B)

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You would like to identify as many bacterial species as possible in the human mouth. Why is biochemical testing not the most efficient and accurate method of completing this task?


A) Since you do not know the growth characteristics and metabolic properties of the bacteria in the mouth, you cannot determine all varieties of media to use for isolation and culture.
B) Bacteriological media is very expensive, particularly when you would have to use a variety of types to identify the different bacteria.
C) Bacteria in the mouth are difficult to grow because of the adverse conditions in the oral habitat.
D) Biochemical testing, using a variety of media, is inaccurate. It has been replaced by more advanced testing procedures.

E) None of the above
F) B) and C)

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