Question 1

When patient tissues are transfected with viruses carrying a needed, normal human gene, the technique is called ______.

Accepted Answer

A)   cloning
B)   gene therapy
C)   antisense therapy
D)   DNA ﬁngerprintingE) A) and B)
F) None of the above

Question 2

The primers in PCR are ______.

Accepted Answer

A)   synthetic DNA oligonucleotides
B)   reverse transcriptases
C)   bacterial enzymes
D)   short RNA strands
E)   DNA polymerasesF) D) and E)
G) C) and D)

Question 3

What type of DNA map is most detailed?

Accepted Answer

A)   Linkage
B)   Sequence
C)   Physical
D)   Geographical
E)   ChromosomalF) All of the above
G) B) and C)

Question 4

An example of gene therapy is the insertion of the gene for human growth hormone into E. coli cells.

Accepted Answer

A) True 
 B)False

Question 5

DNA strands can begin to separate at the temperature of ______.

Accepted Answer

A)   37oC
B)   42oC
C)   60oC
D)   90oC
E)   100oCF) A) and D)
G) C) and D)

Question 6

Genomic _____ are collections of isolated genes maintained in cloning hosts.

Accepted Answer

A)   DNA
B)   libraries
C)   clones
D)   digests
E)   booksF) None of the above
G) A) and B)

Question 7

Restriction endonucleases recognize and clip DNA base sequences called ______.

Accepted Answer

A)   codons
B)   palindromes
C)   introns
D)   exons
E)   genesF) A) and B)
G) A) and C)

Question 8

Which PCR step causes the denaturation of double-stranded DNA?

Accepted Answer

A)   Add DNA polymerase and nucleotides at 72° C.
B)   Cool DNA to between 50° C and 65° C.
C)   Add primers.
D)   Heat target DNA to 94° C.
E)   Repeat the cycle of heating and cooling.F) A) and B)
G) B) and E)

Question 9

E. coli is the best host for cloning because it possesses the mechanisms for processing and modifying proteins.

Accepted Answer

A) True 
 B)False

Question 10

The difference between somatic cell gene therapy and germline therapy is that ______.

Accepted Answer

A)   somatic cell gene therapy is introduced into the egg, sperm or developing embryo, whereas germline therapy introduces a new gene into a mature tissue
B)   somatic cell gene therapy overcomes a protein malfunction in speciﬁc tissues but is not repaired in the entire organism and cannot be passed on to offspring
C)   germline therapy is the temporary repair of a genetic mutation, whereas somatic cell therapy is a permanent ﬁx
D)   germline therapy overcomes a protein malfunction in speciﬁc tissues but is not repaired in the entire organism and cannot be passed on to offspringE) A) and B)
F) A) and C)

Question 11

Each of the following are features of a cloning vector except ______.

Accepted Answer

A)   origin of replication
B)   reverse transcriptases
C)   genetic markers used to screen for recombinants
D)   capacity for large inserts
E)   multiple cloning sitesF) A) and B)
G) B) and E)

Question 12

After three replication cycles in PCR, there will be a total of three double-stranded DNA molecules.

Accepted Answer

A) True 
 B)False

Question 13

Labeled, known, short stretches of DNA used to detect a speciﬁc sequence of nucleotides in a mixture are known as ______.

Accepted Answer

A)   genetic engineering
B)   biotechnology
C)   recombinant DNA
D)   gel electrophoresis
E)   gene probesF) A) and D)
G) A) and E)

Question 14

DNA polymerases used in PCR ______.

Accepted Answer

A)   include Taq polymerases and Vent polymerase
B)   use an RNA template to make complementary DNA
C)   are labeled with ﬂuorescent dyes
D)   must remain active at very cold temperaturesE) C) and D)
F) B) and C)

Question 15

Fluorescent in situ hybridization (FISH)  probes are applied to intact cells and observed microscopically for the presence and location of ______ .

Accepted Answer

A)   RNA
B)   a speciﬁc genetic marker sequence
C)   proteins
D)   recombinant DNA
E)   DNAF) C) and E)
G) None of the above

Question 16

Of the following choices, which could be used in the treatment of a patient in order to determine the patient's cancer subtype?

Accepted Answer

A)   PCR
B)   Oryza sativa
C)   Western Blot analysis
D)   Transformation
E)   Microarray analysisF) C) and D)
G) B) and E)

Question 17

SNPs are derived from ______.

Accepted Answer

A)   point mutations
B)   gene therapy
C)   frameshift mutations
D)   genetic engineeringE) A) and B)
F) C) and D)

Question 18

Common vectors used to transfer a piece of DNA into a cloning host are ______.

Accepted Answer

A)   plasmids
B)   viruses
C)   bacteriophages
D)   artiﬁcial chromosomes
E)   All of the choices are correct.F) A) and E)
G) C) and D)

Question 19

The size of DNA is often given in the number of _____ that it contains.

Accepted Answer

A)   genes
B)   codons
C)   base pairs
D)   proteins
E)   tripletsF) A) and C)
G) A) and E)

Question 20

DNA fragments can be separated in gel electrophoresis because ______.

Accepted Answer

A)   nitrogenous bases have a net negative charge
B)   nitrogenous bases have a net positive charge
C)   phosphate groups have a net positive charge
D)   phosphate groups have a net negative chargeE) A) and C)
F) B) and C)

Exam 10: Genetic Analysis and Genetic Engineering

E. coli is the best host for cloning because it possesses the mechanisms for processing and modifying proteins.

Correct AnswerverifiedShow Answer

Each of the following are features of a cloning vector except ______.

Correct AnswerverifiedShow Answer

DNA fragments can be separated in gel electrophoresis because ______.

Correct AnswerverifiedShow Answer

The primers in PCR are ______.

Correct AnswerverifiedShow Answer

An example of gene therapy is the insertion of the gene for human growth hormone into E. coli cells.

Correct AnswerverifiedShow Answer

Which PCR step causes the denaturation of double-stranded DNA?

Correct AnswerverifiedShow Answer

The difference between somatic cell gene therapy and germline therapy is that ______.

Correct AnswerverifiedShow Answer

SNPs are derived from ______.

Correct AnswerverifiedShow Answer

Genomic _____ are collections of isolated genes maintained in cloning hosts.

Correct AnswerverifiedShow Answer

Labeled, known, short stretches of DNA used to detect a speciﬁc sequence of nucleotides in a mixture are known as ______.

Correct AnswerverifiedShow Answer

Of the following choices, which could be used in the treatment of a patient in order to determine the patient's cancer subtype?

Correct AnswerverifiedShow Answer

Fluorescent in situ hybridization (FISH) probes are applied to intact cells and observed microscopically for the presence and location of ______ .

Correct AnswerverifiedShow Answer

DNA polymerases used in PCR ______.

Correct AnswerverifiedShow Answer

Common vectors used to transfer a piece of DNA into a cloning host are ______.

Correct AnswerverifiedShow Answer

When patient tissues are transfected with viruses carrying a needed, normal human gene, the technique is called ______.

Correct AnswerverifiedShow Answer

DNA strands can begin to separate at the temperature of ______.

Correct AnswerverifiedShow Answer

Restriction endonucleases recognize and clip DNA base sequences called ______.

Correct AnswerverifiedShow Answer

The size of DNA is often given in the number of _____ that it contains.

Correct AnswerverifiedShow Answer

What type of DNA map is most detailed?

Correct AnswerverifiedShow Answer

After three replication cycles in PCR, there will be a total of three double-stranded DNA molecules.

Correct AnswerverifiedShow Answer

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