Question 1

It is possible to identify mRNA molecules using ﬂuorescently labeled cDNA.

Accepted Answer

A) True 
 B)False

Question 2

Which step in gene cloning can occur even more rapidly by PCR?

Accepted Answer

A)   Target DNA removed from cells and isolated
B)   Cloning host treated with calcium chloride and receives plasmid
C)   Target DNA and plasmid treated with the same restriction endonuclease
D)   Desired protein is produced by cloning host
E)   Gene is ampliﬁed by multiplication of cloning hostF) A) and B)
G) A) and C)

Question 3

When micro RNA molecules malfunction, their effect is limited to the inhibition of protein production, which results in tumor growth.

Accepted Answer

A) True 
 B)False

Question 4

The process of introducing a needed, normal gene into human cells is called DNA mapping.

Accepted Answer

A) True 
 B)False

Question 5

Two different nucleic acids can _____ by annealing at their complementary sites.

Accepted Answer

A)   ligate
B)   covalently bond
C)   form a peptide bond
D)   hybridizeE) A) and B)
F) C) and D)

Question 6

It is now possible to very quickly map the genome of an organism or virus.

Accepted Answer

A) True 
 B)False

Question 7

The enzyme required to attach the sticky ends of DNA and used when splicing DNA fragments into other DNA is ______.

Accepted Answer

A)   helicase
B)   ligase
C)   endonuclease
D)   reverse transcriptaseE) A) and C)
F) B) and D)

Question 8

Which of the following is not true of vectors?

Accepted Answer

A)   They are easy to manipulate.
B)   They contain a gene for drug resistance.
C)   An origin of replication (ORI)  is present.
D)   They can detect RNA in cells.
E)   They must accept DNA of desired size.F) A) and C)
G) All of the above

Question 9

Gene probes can be labeled for detection with reporter molecules such as _____.

Accepted Answer

A)   enzymes
B)   ﬂuorescent dyes
C)   radioisotopes
D)   All of the choices can be used.E) B) and D)
F) None of the above

Question 10

Micro RNA therapy is based on the premise that ______.

Accepted Answer

A)   miRNA molecules function to regulate gene expression, usually by inhibiting transcription; a non-functional miRNA can be replaced thereby reducing unwanted protein production
B)   miRNA molecules function to regulate gene expression, usually by promoting translation; a non-functional miRNA can be repaired thereby reducing unwanted protein production
C)   miRNA molecules function to regulate gene expression, usually by initiating transcription; an over-production of miRNA can be replaced thereby promoting necessary proteinProduction
D)   miRNA molecules function to regulate protein production, usually by inhibiting translation; a hyperactive miRNA can be replaced thereby reducing unwanted proteinsE) B) and D)
F) All of the above

Question 11

Humans display how much DNA similarity with mice?

Accepted Answer

A)   50%
B)   60%
C)   70%
D)   80%
E)   90%F) B) and D)
G) A) and E)

Question 12

The various techniques by which scientists manipulate DNA in the lab are termed ______.

Accepted Answer

A)   genetic engineering
B)   biotechnology
C)   recombinant DNA
D)   gel electrophoresis
E)   gene probesF) A) and B)
G) None of the above

Question 13

Your infant has been diagnosed with severe combined immunodeﬁciency disease (SCID) . Your doctor suggests which treatment as a possible cure for the disease?

Accepted Answer

A)   Genome mapping
B)   Reverse transcription
C)   Gene therapy
D)   MicroarraysE) B) and C)
F) A) and B)

Question 14

Any use of an organism's biochemical processes to create a product is referred to as ______.

Accepted Answer

A)   genetic engineering
B)   biotechnology
C)   recombinant DNA
D)   gel electrophoresis
E)   gene probesF) A) and D)
G) B) and E)

Question 15

The deliberate removal of genetic material from one organism and its subsequent transfer into the genome of another organism is a speciﬁc technique called ______.

Accepted Answer

A)   genetic engineering
B)   biotechnology
C)   recombinant DNA technology
D)   gel electrophoresis
E)   gene probe technologyF) D) and E)
G) A) and C)

Question 16

Each of the following are features of a cloning host except ______.

Accepted Answer

A)   quick generation time
B)   minimal growth requirements
C)   mapped genome
D)   pathogenicity
E)   transformableF) A) and B)
G) All of the above

Question 17

Which of the following is a list of the materials required for PCR?

Accepted Answer

A)   Reverse transcriptase, Taq DNA polymerase, nucleotides
B)   Primers, Taq RNA polymerase, nucleotides
C)   Primers, Taq DNA polymerase, nucleotides
D)   Reverse transcriptase, Taq RNA polymerase, nucleotidesE) All of the above
F) A) and D)

Question 18

Transformation and transduction are methods used to introduce DNA into host cells.

Accepted Answer

A) True 
 B)False

Question 19

Why is an enzyme from a thermophilic bacterium used in PCR?

Accepted Answer

A)   It is cheaper to obtain from live microorganisms than producing the enzyme in a lab.
B)   DNA is replicated at a high temperature that denatures most proteins.
C)   The enzyme makes DNA that is more similar to human DNA.
D)   This thermohile's enzyme will synthesize DNA.E) None of the above
F) C) and D)

Question 20

You have made a speci?c DNA probe that will bind to a key sequence on the DNA of Neisseria gonorrhoeae, allowing your company to market a gonorrhea test kit that can be used to identify the bacterium in genital tract specimens. However, upon testing it out against a known Neisseria gonorrhoeae culture, you ?nd that it does not work. Which of the following is a possible explanation for this negative result?

Accepted Answer

A)   You forgot to incubate the test at the 75oC temperature required for hybridization.
B)   You forgot to label the probe sequence with a reporter molecule.
C)   You forgot to add the mRNA to the DNA probe sample.
D)   You forgot to digest the probe with restriction endonucleases.E) A) and B)
F) A) and C)

Exam 10: Genetic Analysis and Genetic Engineering

It is now possible to very quickly map the genome of an organism or virus.

Correct Answer
verified

Correct Answer
verified

Each of the following are features of a cloning host except ______.

Correct Answer
verified

Your infant has been diagnosed with severe combined immunodeﬁciency disease (SCID) . Your doctor suggests which treatment as a possible cure for the disease?

Correct Answer
verified

The various techniques by which scientists manipulate DNA in the lab are termed ______.

Correct Answer
verified

The process of introducing a needed, normal gene into human cells is called DNA mapping.

Correct Answer
verified

Transformation and transduction are methods used to introduce DNA into host cells.

Correct Answer
verified

Which of the following is not true of vectors?

Correct Answer
verified

Which step in gene cloning can occur even more rapidly by PCR?

Correct Answer
verified

Humans display how much DNA similarity with mice?

Correct Answer
verified

The enzyme required to attach the sticky ends of DNA and used when splicing DNA fragments into other DNA is ______.

Correct Answer
verified

Why is an enzyme from a thermophilic bacterium used in PCR?

Correct Answer
verified

The deliberate removal of genetic material from one organism and its subsequent transfer into the genome of another organism is a speciﬁc technique called ______.

Correct Answer
verified

Any use of an organism's biochemical processes to create a product is referred to as ______.

Correct Answer
verified

When micro RNA molecules malfunction, their effect is limited to the inhibition of protein production, which results in tumor growth.

Correct Answer
verified

Micro RNA therapy is based on the premise that ______.

Correct Answer
verified

It is possible to identify mRNA molecules using ﬂuorescently labeled cDNA.

Correct Answer
verified

Which of the following is a list of the materials required for PCR?

Correct Answer
verified

Gene probes can be labeled for detection with reporter molecules such as _____.

Correct Answer
verified

Two different nucleic acids can _____ by annealing at their complementary sites.

Correct Answer
verified

Exam 10: Genetic Analysis and Genetic Engineering

It is now possible to very quickly map the genome of an organism or virus.

Correct AnswerverifiedShow Answer

Correct AnswerverifiedShow Answer

Each of the following are features of a cloning host except ______.

Correct AnswerverifiedShow Answer

Your infant has been diagnosed with severe combined immunodeﬁciency disease (SCID) . Your doctor suggests which treatment as a possible cure for the disease?

Correct AnswerverifiedShow Answer

The various techniques by which scientists manipulate DNA in the lab are termed ______.

Correct AnswerverifiedShow Answer

The process of introducing a needed, normal gene into human cells is called DNA mapping.

Correct AnswerverifiedShow Answer

Transformation and transduction are methods used to introduce DNA into host cells.

Correct AnswerverifiedShow Answer

Which of the following is not true of vectors?

Correct AnswerverifiedShow Answer

Which step in gene cloning can occur even more rapidly by PCR?

Correct AnswerverifiedShow Answer

Humans display how much DNA similarity with mice?

Correct AnswerverifiedShow Answer

The enzyme required to attach the sticky ends of DNA and used when splicing DNA fragments into other DNA is ______.

Correct AnswerverifiedShow Answer

Why is an enzyme from a thermophilic bacterium used in PCR?

Correct AnswerverifiedShow Answer

The deliberate removal of genetic material from one organism and its subsequent transfer into the genome of another organism is a speciﬁc technique called ______.

Correct AnswerverifiedShow Answer

Any use of an organism's biochemical processes to create a product is referred to as ______.

Correct AnswerverifiedShow Answer

When micro RNA molecules malfunction, their effect is limited to the inhibition of protein production, which results in tumor growth.

Correct AnswerverifiedShow Answer

Micro RNA therapy is based on the premise that ______.

Correct AnswerverifiedShow Answer

It is possible to identify mRNA molecules using ﬂuorescently labeled cDNA.

Correct AnswerverifiedShow Answer

Which of the following is a list of the materials required for PCR?

Correct AnswerverifiedShow Answer

Gene probes can be labeled for detection with reporter molecules such as _____.

Correct AnswerverifiedShow Answer

Two different nucleic acids can _____ by annealing at their complementary sites.

Correct AnswerverifiedShow Answer

Correct Answer
verified

Correct Answer
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Correct Answer
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Correct Answer
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Correct Answer
verified

Correct Answer
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Correct Answer
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Correct Answer
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