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You are working in a laboratory that is studying a newly isolated virus. Your job is to culture the virus using in vitro methods. Upon observing your inoculated tissue culture specimen one day, you notice clumps of cells growing on top of the original monolayer of cells. Microscopic analysis of stained cells from the culture reveal an alteration in host cell membrane protein content and chromosomal structure. Based upon this information, you hypothesize that the virus you are studying is ______.


A) a bacteriophage
B) a prion
C) a viroid
D) an oncovirus
E) a satellite virus

F) C) and E)
G) A) and E)

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Viruses are simple, noncellular, lacking mRNA and ribosomes.

A) True
B) False

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Satellite viruses are _______.


A) also called viroids
B) dependent on other viruses for replication
C) the cause of spongiform encephalopathies
D) signi?cant pathogens of plants

E) B) and C)
F) All of the above

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Viruses are unable to multiply outside of a host cell.

A) True
B) False

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True

Viruses that cause infection resulting in alternating periods of activity with symptoms and inactivity without symptoms are called ______.


A) latent
B) oncogenic
C) prions
D) viroids
E) delta agents

F) None of the above
G) B) and D)

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What structures are used by bacteriophages to attach to host cell receptors?


A) Viral sheaths
B) Tail fibers
C) Nucleic acids
D) Capsid heads

E) A) and C)
F) All of the above

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Viral growth in bird embryos can cause discrete, opaque spots in the embryonic membranes called ______.


A) patches
B) buds
C) plaques
D) cytopathic effects
E) pocks

F) B) and D)
G) None of the above

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A common method for cultivating viruses in the lab is to use in vitro systems called _____ cultures.


A) embryo
B) cell
C) plaque
D) bacteriophage
E) egg

F) D) and E)
G) A) and B)

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B

T-even phages ______.


A) include the poxviruses
B) infect Escherichia coli cells
C) enter host cells by engulfment
D) have helical capsids

E) C) and D)
F) All of the above

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During lysogeny, an inactive prophage state occurs when the viral DNA is inserted into the host ______.


A) cytoplasm
B) nucleus
C) nucleolus
D) DNA
E) cell membrane

F) B) and C)
G) A) and C)

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The process of dissolving the envelope and capsid to release the viral nucleic acid is ______.


A) adsorption
B) penetration
C) uncoating
D) synthesis
E) assembly

F) A) and B)
G) A) and C)

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C

Viruses attach to their hosts via ______.


A) host glycoproteins
B) host phospholipids
C) viral phospholipids
D) viral flagella
E) carbohydrate attachments of the viral capsid

F) A) and D)
G) A) and C)

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Successful anti-HIV drug therapies today work by blocking the action of viral reverse transcriptase. Select which step of virus multiplication that would be directly blocked by this mechanism.


A) Adsorption
B) Penetration
C) Synthesis
D) Assembly
E) Release

F) C) and D)
G) None of the above

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In order to replicate within a host cell, a virus with a genome comprised of single-stranded positive- sense RNA _______.


A) must use its genomic strand as a template to make copies of negative-sense RNA for packaging
B) must use its genomic strand as a template to make copies of positive-sense RNA for packaging
C) must first use its genomic strand as a template to make a negative-sense RNA, which then serves as a template to synthesize positive-sense RNA for packaging
D) must first use its genomic strand as a template to make copies of DNA for packaging

E) B) and D)
F) None of the above

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Viruses ______.


A) cannot be seen in a light microscope
B) are prokaryotic
C) contain 70S ribosomes
D) undergo binary ?ssion

E) A) and B)
F) A) and C)

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All of the following pertain to virus envelopes except ________.


A) gained as a virus leaves the host cell membrane
B) gained as a virus leaves the nuclear membrane
C) contain special virus proteins
D) help the virus particle attach to host cells
E) located between the capsid and nucleic acid

F) B) and D)
G) A) and E)

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Viral spikes are inserted into the host cell membrane before budding or exocytosis.

A) True
B) False

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Viral nomenclature uses the same system as living organisms; a genus and speci?c epithet is designated for each virus.

A) True
B) False

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Viruses acquire envelopes around their nucleocapsids during ______.


A) replication
B) assembly
C) adsorption
D) release
E) penetration

F) All of the above
G) B) and C)

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The envelope of enveloped viruses _______.


A) is identical to the host plasma membrane
B) is only composed of host endomembrane
C) does not contain spikes
D) is obtained by viral budding or exocytosis
E) makes the virus very susceptible to drug therapy

F) A) and E)
G) A) and D)

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