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Both gram-positive and gram-negative cells have outer membranes.

A) True
B) False

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Which of the following properties are shared by both bacteria and archaea?


A) Single, circular chromosome
B) Cell wall polysaccharides
C) Ester linkages in fatty acids
D) Method of DNA compaction

E) A) and B)
F) C) and D)

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The macromolecule containing alternating N-acetylglucosamine (NAG) and N-acetylmuramic acid (NAM) chains cross-linked by short peptide fragments is ______.


A) teichoic acid
B) peptidoglycan
C) lipopolysaccharide
D) mycolic acid
E) lysozyme

F) C) and D)
G) B) and D)

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The site for ATP synthesis in bacterial cells is the ______.


A) cell membrane
B) ribosome
C) mitochondrion
D) cell wall
E) microcompartment

F) None of the above
G) All of the above

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You've just isolated a new bacterium in pure culture, and you culture it on a general purpose medium where its cells have a coccobacillus morphology. When you examine the cells after culturing on a variety of differential media, you ?nd that in some cases, the cells appear a coccobacillus, but in others, they can be ?lamentous, cocci, or club-shaped. The best explanation for this observation is that _______.


A) the media were incubated at an incorrect temperature
B) the microscope is out of adjustment
C) the differential media are contaminated
D) the bacterium is pleomorphic
E) your culture has become contaminated

F) B) and D)
G) B) and E)

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A slime layer gives bacterial cells greater pathogenicity than a capsule.

A) True
B) False

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The transfer of genes during bacterial conjugation involves rigid, tubular appendages called ______.


A) fimbriae
B) cilia
C) flagella
D) sex pili
E) periplasmic flagella (axial filaments)

F) A) and D)
G) None of the above

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The term that refers to the presence of flagella all over the cell surface is ______.


A) atrichous
B) peritrichous
C) monotrichous
D) amphitrichous
E) lophotrichous

F) C) and D)
G) B) and E)

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All bacterial cells have ______.


A) capsules
B) flagella
C) a chromosome
D) the ability to produce endospores
E) an S-layer

F) All of the above
G) A) and B)

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The cell envelope of gram-positive bacteria has two layers: a thick cell wall and the cell membrane.

A) True
B) False

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Which external structure protects bacteria from phagocytosis?


A) Slime layer
B) Cell membrane
C) Capsule
D) Fimbriae

E) C) and D)
F) None of the above

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The term that refers to flagella at both poles of the cell is ______.


A) peritrichous
B) atrichous
C) amphitrichous
D) monotrichous
E) lophotrichous

F) B) and C)
G) A) and B)

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The basal body of a flagellum is anchored into the ______.


A) cell wall
B) outer membrane
C) cell membrane
D) peptidoglycan layer
E) hook

F) D) and E)
G) B) and E)

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Boiling water (100°C) can normally destroy endospores.

A) True
B) False

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Lysozyme is most effective against ______.


A) gram-positive organisms
B) mycoplasmas
C) gram-negative organisms
D) cyanobacteria
E) archaea

F) D) and E)
G) A) and C)

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You've just isolated a new bacterium in pure culture, and you culture it on a general purpose medium where its cells have a rod-shape (bacillus) morphology. In addition to culturing it on solid media, you inoculate a slide to grow it as a bio?lm. When you use confocal microscopy to image the bio?lm, you observe that there are several cellular morphologies, ranging from coccus to coccobacillus to long bacillus. The best explanation for this observation is that _______.


A) the bio?lm is no longer a pure culture of the microbe
B) the bio?lm culture was not incubated under the appropriate conditions
C) the microenvironments within a bio?lm promote structural variation
D) the confocal microscope is out of adjustment

E) C) and D)
F) A) and B)

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Gram-negative bacteria _______.


A) have a more complex cell envelope with a greater variation in chemical composition
B) appear purple following the Gram stain
C) are generally more susceptible to antibiotics than gram-positive bacteria
D) include all pathogens

E) A) and B)
F) A) and C)

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Chemotaxis refers to the ability of a cell to _______.


A) move in response to a chemical
B) halt movement in response to a chemical
C) transport desired molecules into a cell
D) move in response to light

E) A) and B)
F) C) and D)

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Which of the following statements regarding chemotaxis is incorrect?


A) In a cell with peritrichous flagella, each flagellum moves individually but in a coordinated direction during a run.
B) In a tumble, the flagellum reverses direction, causing the cell to stop moving and change course.
C) The fuel for flagellar motion is not ATP, but a proton gradient.
D) When a cell detects a nutrient gradient, it will increase the frequency of runs to move toward the nutrient.

E) A) and D)
F) All of the above

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Archaea have been found in many microenvironments in the human body. Which of the following microenvironments is most likely to fit with the designation of Archaea as "extremophiles?"


A) The gingiva and the gut are anaerobic.
B) The inner ear can withstand increased pressure.
C) The temperature of the testes is less than 37°C.
D) The surface of the tongue has salt receptors.

E) C) and D)
F) All of the above

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