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Mycobacterium and Nocardia are different from most gram-positive bacteria in that their cell walls _______.


A) are easily decolorized during staining
B) contain unique, waxy lipids
C) contain a layer of lipopolysaccharide
D) contain more peptidoglycan

E) A) and C)
F) B) and C)

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A bacterial cell wall that has primarily peptidoglycan with small amounts of teichoic acid and lipoteichoic acid is ______.


A) gram-positive
B) a protoplast
C) a spheroplast
D) gram-negative
E) acid-fast

F) A) and B)
G) B) and E)

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A bacterial genus that has waxy mycolic acid in the cell walls is ______.


A) Bacillus
B) Corynebacterium.
C) Mycobacterium
D) Streptococcus
E) Mycoplasma

F) B) and C)
G) A) and B)

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Hot carbol fuchsin is the primary dye in the acid-fast stain.

A) True
B) False

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You have made a smear of a bacterial culture and have performed the Gram stain on it. Looking at the organism under the microscope, you notice that the cells do not seem to be the dark blue- Purple of a gram-positive reaction, but instead are light purple. Your staining procedure was Performed correctly. What is your best explanation as to why the bacteria have stained this way?


A) The specimen did not undergo heat ?xation before staining; therefore, the primary dye does not stick properly to the wall.
B) These are mutant bacteria which cannot be stained like other bacteria.
C) There is something in the wall of the bacteria that has affected the uptake of the crystal violet into the cell wall, thereby staining it improperly.
D) The bacteria were taken from an inappropriate medium, and a chemical is interfering with proper staining of the cells.

E) A) and D)
F) A) and C)

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Which of the following is not a phenotypic trait of bacteria?


A) rRNA sequence
B) Nutrient requirements
C) Biochemical reactions
D) Cell shape

E) B) and C)
F) B) and D)

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Alcohol-based hand sanitizers specifically target lipids, making them most effective when trying to eliminate ______.


A) endospores
B) viruses
C) gram-positives
D) biofilms
E) gram-negatives

F) B) and E)
G) C) and D)

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A nutrient binds to receptors near the flagellar basal body. This will result in _______.


A) inhibition of flagella rotation
B) clockwise rotation of flagella
C) counterclockwise rotation of flagella
D) numerous tumbles

E) All of the above
F) C) and D)

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Endospores of certain bacterial species can enter tissues in the human body, germinate, and cause an infectious disease.

A) True
B) False

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You apply the acid-fast stain method to a patient's specimen, an aspirate from the lungs. Microscopic examination reveals a large number of bright pink-red bacillus-shaped bacteria in the Smear. Which statement is true?


A) This is the expected outcome for normal sputum.
B) The patient has pneumonia.
C) The patient has tuberculosis.
D) The patient has an HIV infection.

E) A) and B)
F) None of the above

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Researchers studying the rigidity and fexibility of the cell envelope used chemotaxis to measure the ability of microbes to squeeze through tight spaces. E. coli, a gram-negative bacterium, was able to traverse narrower channels than B. subtilis, a gram-positive microbe, even though both cells are the same size when grown without restriction. E. coli has the more fexible cell envelope because _______.


A) it has fewer flagella
B) there are fewer protein receptors
C) it has only a single membrane
D) lipopolysaccharide has no charge
E) there is less structural carbohydrate

F) D) and E)
G) A) and C)

Correct Answer

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Two structures that allow bacteria to adhere to surfaces are ______ and ______.


A) actin filaments; phospholipid membranes
B) pili; ribosomes
C) fimbriae; capsules
D) endospores; metachromatic granules
E) lipopolysaccharide; techoic acid

F) D) and E)
G) None of the above

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Peptidoglycan is a unique macromolecule found in bacterial ______.


A) cell membranes
B) cell walls
C) capsules
D) slime layers
E) inclusions

F) A) and B)
G) A) and C)

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Which of the following bacterial structures is incorrectly matched with a function?


A) Ribosomes - protein synthesis
B) Cytoplasm - dense, gelatinous solution
C) Nucleoid - hereditary material
D) Plasmids - contain genes essential for growth and metabolism
E) Inclusions - excess cell nutrients and materials

F) C) and D)
G) D) and E)

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