Question 1

The purpose of staining cells on a microscope slide is to ______.

Accepted Answer

A)   kill them
B)   enlarge the cells
C)   secure them to the slide
D)   observe motility
E)   add contrast to see them betterF) D) and E)
G) A) and E)

Question 2

The term that refers to the purposeful addition of microorganisms into a laboratory nutrient medium is ______.

Accepted Answer

A)   isolation
B)   contamination
C)   inoculation
D)   immunization
E)   infectionF) A) and B)
G) A) and C) C

Question 3

Which method often results in colonies developing down throughout the agar along with some colonies on the surface?

Accepted Answer

A)   Spread plate
B)   Streak plate
C)   Replica plate
D)   Pour plateE) A) and B)
F) A) and C)

Question 4

Which of the following is not a beneﬁt of agar as a solid medium?

Accepted Answer

A)   Can be inoculated and poured at a temperature that is not harmful
B)   Flexibility
C)   Holds moisture
D)   Solid at room temperature
E)   Easily digested by most microbesF) D) and E)
G) B) and C)

Question 5

The three physical forms of laboratory media are ______.

Accepted Answer

A)   reducing, transport, and enumeration
B)   a streak plate, a pour plate, and a broth
C)   solid, liquid, and gas
D)   solid, semisolid, and liquidE) B) and C)
F) B) and D) D

Question 6

A nutrient medium that contains at least one ingredient that is not chemically deﬁned is termed ______.

Accepted Answer

A)   synthetic
B)   enriched
C)   complex
D)   reducingE) A) and C)
F) B) and C)

Question 7

Agar is an important component of media because _______.

Accepted Answer

A)   agar provides a solid surface for bacterial growth
B)   agar prevents contamination
C)   agar inhibits mold growth
D)   bacteria require agar as a source of carbonE) None of the above
F) A) and B)

Question 8

Comparing transmission electron microscopy with scanning electron microscopy, the following statement is true.

Accepted Answer

A)   Transmission EM is used for internal detail of cells and subcellular structures.
B)   Transmission EM can provide good images of bacteria but not viruses.
C)   Transmission EM requires dyes.
D)   Transmission EM gives detail of the external architecture of cells.E) B) and C)
F) A) and B) A

Question 9

Who invented the ?rst crude microscope by grinding glass?

Accepted Answer

A)   Redi
B)   Pasteur
C)   Leeuwenhoek
D)   Lister
E)   Schultz and SchwannF) C) and D)
G) A) and E)

Question 10

Thioglycolate medium contains the following: yeast extract, pancreatic digest of casein, glucose, L- cysteine, sodium chloride, sodium thioglycolate, and agar. It is considered a complex medium because _______.

Accepted Answer

A)   thioglycolate has a carbon-sulfur bond that many microbes cannot break enzymatically
B)   it has multiple ingredients for a broad range of nutrients
C)   both yeast extract and pancreatic digest of casein are not chemically de?ned
D)   depending on their oxygen utilization pattern, organisms will grow differently on itE) None of the above
F) A) and D)

Question 11

The simple microscopes used by the earliest practitioners of microscopy contained which of the following?

Accepted Answer

A)   Magnifying lens
B)   Ocular
C)   Oil-immersion lens
D)   Condenser
E)   LampF) A) and E)
G) B) and E)

Question 12

Which term refers to the microscope's ability to show two separate objects as discrete and distinct?

Accepted Answer

A)   Refraction
B)   Magniﬁcation
C)   Resolution
D)   ContrastE) B) and C)
F) C) and D)

Question 13

The _____ of the microscope holds and allows selection of the objective lenses.

Accepted Answer

A)   ocular
B)   nosepiece
C)   stage
D)   condenserE) A) and B)
F) C) and D)

Question 14

What do the Gram stain, the acid-fast stain, and the endospore stain have in common?

Accepted Answer

A)   They use a negative stain technique.
B)   They use heat to force the dye into cell structures.
C)   They are differential stains.
D)   They are used on a wet mount of the specimen.
E)   The staining outcome varies with the charge of the cell wall.F) A) and C)
G) C) and D)

Question 15

Newly inoculated cultures must be _____ at a specific temperature and time to encourage growth.

Accepted Answer

A)   streaked
B)   poured
C)   incubated
D)   spreadE) C) and D)
F) A) and B)

Question 16

Following a properly-performed Gram stain, gram-positive bacteria will appear as pink or red cells.

Accepted Answer

A) True 
 B)False

Question 17

Which isolation technique is most effective for the majority of applications and is most commonly used for colony isolation in the laboratory?

Accepted Answer

A)   Loop dilution
B)   Streak plate
C)   Pour plate
D)   Spread plate
E)   Culture plateF) B) and E)
G) None of the above

Question 18

All of the following pertain to the ﬂuorescence microscope except _______.

Accepted Answer

A)   it uses electrons to produce a specimen image
B)   it requires the use of dyes like acridine or ﬂuorescein
C)   it requires an ultraviolet radiation source
D)   it is a type of compound microscope
E)   it is used to diagnose certain infectionsF) A) and E)
G) D) and E)

Question 19

A microbiology student with a visual disability is viewing a sample of Bacillus endospores in a phase contrast microscope that is linked to an iPad. By "stretching" the image on the iPad screen, the student can increase the apparent size of the endospores. How does this manipulation affect
Resolution and contrast? (No software is being used to alter the image!)

Accepted Answer

A)   Only the magni?cation can be increased; resolution and contrast are unchanged.
B)   Contrast will improve because the microscope is phase-contrast.
C)   Both magni?cation and resolution increase; contrast is unchanged.
D)   Resolution and magni?cation will decrease because the screen is not as good as the human eye.E) None of the above
F) A) and C)

Question 20

Which type of microscopy transforms the subtle variations in cell density into differences in light intensity, allowing for increased internal detail without staining?

Accepted Answer

A)   Electron
B)   Bright-ﬁeld
C)   Fluorescence
D)   Confocal
E)   Phase-contrastF) A) and E)
G) B) and E)

Exam 3: Tools of the Laboratory: Methods for the Culturing of Microscopic Analysis of Microorganisms

The purpose of staining cells on a microscope slide is to ______.

Correct Answer
verified

The term that refers to the purposeful addition of microorganisms into a laboratory nutrient medium is ______.

Correct Answer
verified
C

Which method often results in colonies developing down throughout the agar along with some colonies on the surface?

Correct Answer
verified

Which of the following is not a beneﬁt of agar as a solid medium?

Correct Answer
verified

The three physical forms of laboratory media are ______.

Correct Answer
verified
D

A nutrient medium that contains at least one ingredient that is not chemically deﬁned is termed ______.

Correct Answer
verified

Agar is an important component of media because _______.

Correct Answer
verified

Comparing transmission electron microscopy with scanning electron microscopy, the following statement is true.

Correct Answer
verified
A

Who invented the ?rst crude microscope by grinding glass?

Correct Answer
verified

Thioglycolate medium contains the following: yeast extract, pancreatic digest of casein, glucose, L- cysteine, sodium chloride, sodium thioglycolate, and agar. It is considered a complex medium because _______.

Correct Answer
verified

The simple microscopes used by the earliest practitioners of microscopy contained which of the following?

Correct Answer
verified

Which term refers to the microscope's ability to show two separate objects as discrete and distinct?

Correct Answer
verified

The _____ of the microscope holds and allows selection of the objective lenses.

Correct Answer
verified

What do the Gram stain, the acid-fast stain, and the endospore stain have in common?

Correct Answer
verified

Newly inoculated cultures must be _____ at a specific temperature and time to encourage growth.

Correct Answer
verified

Following a properly-performed Gram stain, gram-positive bacteria will appear as pink or red cells.

Correct Answer
verified

Which isolation technique is most effective for the majority of applications and is most commonly used for colony isolation in the laboratory?

Correct Answer
verified

All of the following pertain to the ﬂuorescence microscope except _______.

Correct Answer
verified

Correct Answer
verified

Which type of microscopy transforms the subtle variations in cell density into differences in light intensity, allowing for increased internal detail without staining?

Correct Answer
verified

Exam 3: Tools of the Laboratory: Methods for the Culturing of Microscopic Analysis of Microorganisms

The purpose of staining cells on a microscope slide is to ______.

Correct AnswerverifiedShow Answer

The term that refers to the purposeful addition of microorganisms into a laboratory nutrient medium is ______.

Correct AnswerverifiedC

Which method often results in colonies developing down throughout the agar along with some colonies on the surface?

Correct AnswerverifiedShow Answer

Which of the following is not a beneﬁt of agar as a solid medium?

Correct AnswerverifiedShow Answer

The three physical forms of laboratory media are ______.

Correct AnswerverifiedD

A nutrient medium that contains at least one ingredient that is not chemically deﬁned is termed ______.

Correct AnswerverifiedShow Answer

Agar is an important component of media because _______.

Correct AnswerverifiedShow Answer

Comparing transmission electron microscopy with scanning electron microscopy, the following statement is true.

Correct AnswerverifiedA

Who invented the ?rst crude microscope by grinding glass?

Correct AnswerverifiedShow Answer

Thioglycolate medium contains the following: yeast extract, pancreatic digest of casein, glucose, L- cysteine, sodium chloride, sodium thioglycolate, and agar. It is considered a complex medium because _______.

Correct AnswerverifiedShow Answer

The simple microscopes used by the earliest practitioners of microscopy contained which of the following?

Correct AnswerverifiedShow Answer

Which term refers to the microscope's ability to show two separate objects as discrete and distinct?

Correct AnswerverifiedShow Answer

The _____ of the microscope holds and allows selection of the objective lenses.

Correct AnswerverifiedShow Answer

What do the Gram stain, the acid-fast stain, and the endospore stain have in common?

Correct AnswerverifiedShow Answer

Newly inoculated cultures must be _____ at a specific temperature and time to encourage growth.

Correct AnswerverifiedShow Answer

Following a properly-performed Gram stain, gram-positive bacteria will appear as pink or red cells.

Correct AnswerverifiedShow Answer

Which isolation technique is most effective for the majority of applications and is most commonly used for colony isolation in the laboratory?

Correct AnswerverifiedShow Answer

All of the following pertain to the ﬂuorescence microscope except _______.

Correct AnswerverifiedShow Answer

Correct AnswerverifiedShow Answer

Which type of microscopy transforms the subtle variations in cell density into differences in light intensity, allowing for increased internal detail without staining?

Correct AnswerverifiedShow Answer

Correct Answer
verified

Correct Answer
verified
C

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified
D

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified
A

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified