Question 1

In choosing the vector used to carry human genes into a host cell, which of the following should be the important consideration?

Accepted Answer

A)  How much donor DNA can be carried on the vector
B)  What kind of plasmid it is
C)  Whether the vector comes from a bacterium or a virus
D)  Whether the vector contains RNA or DNAE) None of the above
F) B) and C)

Question 2

What type of DNA map is most detailed?

Accepted Answer

A)  Linkage
B)  Sequence
C)  Physical
D)  Geographical
E)  ChromosomalF) A) and B)
G) C) and E)

Question 3

Which of the following is the second step in gene mapping?

Accepted Answer

A)  Target DNA removed from cells and isolated
B)  Cloning host treated with calcium chloride and receives plasmid
C)  Separate DNA fragments with gel electrophoresis
D)  Desired protein is produced by cloning host
E)  Gene is amplified by multiplication of cloning hostF) A) and E)
G) None of the above

Question 4

Genomic _____ are collections of isolated genes maintained in cloning hosts.

Accepted Answer

A)  DNA
B)  libraries
C)  clones
D)  digests
E)  booksF) A) and B)
G) B) and C)

Question 5

DNA polymerases used in PCR

Accepted Answer

A)  use an RNA template to make complementary DNA.
B)  must remain active at very cold temperatures.
C)  include Taq polymerases and Vent polymerase.
D)  are labeled with fluorescent dyes.E) All of the above
F) A) and B)

Question 6

The commercial product Frostban consists of a genetically altered bacterium which prevents ice crystals from forming on plants, thereby reducing freezing of plants and financial distress to the farmers as a result of freezing weather.This product contains a strain of ______.

Accepted Answer

A)  Escherichia coli
B)  Saccharomyces cerevisiae
C)  Thermus aquaticus
D)  Pseudomonas fluorescens
E)  Pseudomonas syringaeF) A) and E)
G) B) and C)

Question 7

Restriction endonucleases are obtained from various species of bacteria.

Accepted Answer

A) True 
 B)False

Question 8

Which of the following is not true of vectors?

Accepted Answer

A)  They are easy to manipulate.
B)  They can detect RNA in cells.
C)  An origin of replication (ORI)  is present.
D)  They contain a gene for drug resistance.
E)  They must accept DNA of desired size.F) All of the above
G) A) and B)

Question 9

The enzyme required to attach the sticky ends of DNA and used when splicing DNA fragments into other DNA is ______.

Accepted Answer

A)  endonuclease
B)  ligase
C)  reverse transcriptase
D)  helicaseE) A) and B)
F) B) and D)

Question 10

The study of genomes of a particular community is called ______.

Accepted Answer

A)  metabolomics
B)  proteomics
C)  genomology
D)  genomics
E)  metagenomicsF) B) and C)
G) C) and E)

Question 11

Any use of an organism's biochemical processes to create a product is referred to as ______.

Accepted Answer

A)  genetic engineering
B)  biotechnology
C)  recombinant DNA
D)  gel electrophoresis
E)  gene probesF) None of the above
G) B) and D)

Question 12

Which PCR step causes the denaturation of double-stranded DNA?

Accepted Answer

A)   Add DNA polymerase and nucleotides at 72° C.
B)   Cool DNA to between 50° C and 65° C.
C)   Add primers.
D)   Heat target DNA to 94° C.
E)   Repeat the cycle of heating and cooling.F) A) and E)
G) None of the above

Question 13

Each of the following are features of a cloning vector except

Accepted Answer

A)  origin of replication.
B)  reverse transcriptases.
C)  genetic markers used to screen for recombinants.
D)  capacity for large inserts.
E)  multiple cloning sites.F) B) and E)
G) A) and B)

Question 14

Of the following choices, which could be used in the treatment of a patient in order to determine the patient's cancer subtype?

Accepted Answer

A)  Western Blot analysis
B)  PCR
C)  Microarray analysis
D)  Transformation
E)  Oryza sativaF) A) and E)
G) B) and E)

Question 15

Humans display how much DNA similarity with mice?

Accepted Answer

A)  50%
B)  60%
C)  70%
D)  80%
E)  90%F) B) and D)
G) D) and E)

Question 16

Which of the following techniques is mismatched with its use in DNA fingerprinting?

Accepted Answer

A)  PCR amplification to get more copies of DNA
B)  Hybridization probes to digest the DNA sample
C)  Electrophoresis to separate DNA fragments
D)  Southern blot for a visual record of DNA fragments
E)  Restriction endonucleases to cut DNAF) B) and E)
G) C) and E)

Question 17

Fluorescent in situ hybridization (FISH)  probes are applied to intact cells and observed microscopically for the presence and location of

Accepted Answer

A)  DNA.
B)  proteins.
C)  a specific genetic marker sequence.
D)  recombinant DNA.
E)  RNA.F) A) and E)
G) A) and D)

Question 18

Vectors often contain a gene conferring drug resistance to their cloning host, in order to detect cells harboring the plasmid.

Accepted Answer

A) True 
 B)False

Question 19

Genetically modified organisms include _____.

Accepted Answer

A)  bacteria
B)  viruses
C)  plants
D)  nonhuman animals
E)  All of the above have been genetically modified.F) A) and B)
G) B) and D)

Question 20

Reverse transcriptase is used to make cDNA from an RNA template.

Accepted Answer

A) True 
 B)False

Exam 10: Genetic Engineering and Recombinant Dna

The enzyme required to attach the sticky ends of DNA and used when splicing DNA fragments into other DNA is ______.

Correct AnswerverifiedShow Answer

Which of the following is not true of vectors?

Correct AnswerverifiedShow Answer

What type of DNA map is most detailed?

Correct AnswerverifiedShow Answer

Restriction endonucleases are obtained from various species of bacteria.

Correct AnswerverifiedShow Answer

The study of genomes of a particular community is called ______.

Correct AnswerverifiedShow Answer

DNA polymerases used in PCR

Correct AnswerverifiedShow Answer

In choosing the vector used to carry human genes into a host cell, which of the following should be the important consideration?

Correct AnswerverifiedShow Answer

Of the following choices, which could be used in the treatment of a patient in order to determine the patient's cancer subtype?

Correct AnswerverifiedShow Answer

Humans display how much DNA similarity with mice?

Correct AnswerverifiedShow Answer

Any use of an organism's biochemical processes to create a product is referred to as ______.

Correct AnswerverifiedShow Answer

Fluorescent in situ hybridization (FISH) probes are applied to intact cells and observed microscopically for the presence and location of

Correct AnswerverifiedShow Answer

The commercial product Frostban consists of a genetically altered bacterium which prevents ice crystals from forming on plants, thereby reducing freezing of plants and financial distress to the farmers as a result of freezing weather.This product contains a strain of ______.

Correct AnswerverifiedShow Answer

Genetically modified organisms include _____.

Correct AnswerverifiedShow Answer

Which of the following techniques is mismatched with its use in DNA fingerprinting?

Correct AnswerverifiedShow Answer

Reverse transcriptase is used to make cDNA from an RNA template.

Correct AnswerverifiedShow Answer

Which of the following is the second step in gene mapping?

Correct AnswerverifiedShow Answer

Genomic _____ are collections of isolated genes maintained in cloning hosts.

Correct AnswerverifiedShow Answer

Each of the following are features of a cloning vector except

Correct AnswerverifiedShow Answer

Which PCR step causes the denaturation of double-stranded DNA?

Correct AnswerverifiedShow Answer

Vectors often contain a gene conferring drug resistance to their cloning host, in order to detect cells harboring the plasmid.

Correct AnswerverifiedShow Answer

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