Question 1

A microbiologist makes a fixed smear of bacterial cells and stains it with methylene blue.All cells appear blue under the oil immersion lens.This is an example of ______.

Accepted Answer

A)  simple staining
B)  negative staining
C)  capsule staining
D)  the acid-fast stain
E)  using an acidic dyeF) A) and B)
G) B) and D)

Question 2

Which type of microscope cannot image live specimens?

Accepted Answer

A)  Differential interference
B)  Bright-field
C)  Electron
D)  Dark-field
E)  Phase-contrastF) All of the above
G) B) and C)

Question 3

Which of the following is not a benefit of agar as a solid medium?

Accepted Answer

A)  Holds moisture
B)  Flexibility
C)  Solid at room temperature
D)  Can be inoculated and poured at a temperature that is not harmful
E)  Easily digested by most microbesF) A) and D)
G) B) and E)

Question 4

All of the following are diameters of cells that would be resolved in a microscope with a limit of resolution of 0.2 µm except ______.

Accepted Answer

A)  0.2 mm
B)  0.1 µm
C)  2.0 µm
D)  0.2 µmE) None of the above
F) C) and D)

Question 5

A common medium for culturing fastidious bacteria is ______.

Accepted Answer

A)  trypticase soy agar
B)  reducing medium
C)  mannitol salt agar
D)  blood agar
E)  MacConkey mediumF) C) and E)
G) C) and D)

Question 6

Which type of medium distinguishes different types of microorganisms based on an observable change in the colonies or in the medium?

Accepted Answer

A)  Enriched
B)  Enumeration
C)  Selective
D)  Reducing
E)  DifferentialF) B) and E)
G) D) and E)

Question 7

What is the term for a culture made from one isolated colony?

Accepted Answer

A)  Axenic
B)  Mixed
C)  Pure
D)  Both axenic and mixed are correct.
E)  Both axenic and pure are correct.F) B) and D)
G) B) and C)

Question 8

Which of the following parts of the microscope magnifies the specimen to produce the virtual image of the specimen?

Accepted Answer

A)  Ocular lens
B)  Objective lens
C)  Condenser
D)  BodyE) A) and D)
F) C) and D)

Question 9

Which of the following parts of a microscope magnifies the specimen to produce a real image of the specimen?

Accepted Answer

A)  Body
B)  Condenser
C)  Objective lens
D)  Ocular lens
E)  NosepieceF) A) and D)
G) None of the above

Question 10

The bending of light rays as they pass from one medium to another is called refraction.

Accepted Answer

A) True 
 B)False

Question 11

The term that refers to the purposeful addition of microorganisms into a laboratory nutrient medium is ______.

Accepted Answer

A)  inoculation
B)  contamination
C)  infection
D)  immunization
E)  isolationF) A) and E)
G) None of the above

Question 12

The procedures for culturing a microorganism require the use of a microscope.

Accepted Answer

A) True 
 B)False

Question 13

Which type of microscope achieves the greatest resolution and highest magnification?

Accepted Answer

A)  Bright-field
B)  Fluorescence
C)  Electron
D)  Dark-field
E)  Phase-contrastF) None of the above
G) All of the above

Question 14

Which type of medium would be the best choice when shipping a sample of bacteria from a clinic to a laboratory for testing?

Accepted Answer

A)  Thioglycolate medium
B)  Eosin-methylene blue agar
C)  Transport medium
D)  Blood agarE) B) and C)
F) B) and D)

Question 15

Which term refers to the microscope's ability to show two separate objects as discrete and distinct?

Accepted Answer

A)  Contrast
B)  Magnification
C)  Refraction
D)  ResolutionE) A) and D)
F) A) and B)

Question 16

What do the Gram stain, the acid-fast stain, and the endospore stain have in common?

Accepted Answer

A)  They use heat to force the dye into cell structures.
B)  The staining outcome varies with the charge of the cell wall.
C)  They are used on a wet mount of the specimen.
D)  They are differential stains.
E)  They use a negative stain technique.F) A) and B)
G) D) and E)

Question 17

Which type of microscope bombards a whole, metal-coated specimen with electrons moving back and forth over it?

Accepted Answer

A)  Transmission electron
B)  Differential interference contrast
C)  Scanning electron
D)  Phase-contrast
E)  FluorescenceF) A) and C)
G) A) and B)

Question 18

Agar is an important component of media because

Accepted Answer

A)  agar provides a solid surface for bacterial growth.
B)  bacteria require agar as a source of carbon.
C)  agar inhibits mold growth.
D)  agar prevents contamination.E) None of the above
F) B) and D)

Question 19

The correct microbiological term for the sample of specimen that is put into a nutrient medium to produce a culture is ______.

Accepted Answer

A)  loop
B)  inoculum
C)  streak
D)  colonyE) A) and D)
F) B) and C)

Question 20

Some microbes are not capable of growing on artificial media as pure cultures.

Accepted Answer

A) True 
 B)False

Exam 3: Tools of the Laboratory: Methods for the Culturing of Microscopic Analysis of Microorganisms

Which type of medium would be the best choice when shipping a sample of bacteria from a clinic to a laboratory for testing?

Correct AnswerverifiedShow Answer

Which type of medium distinguishes different types of microorganisms based on an observable change in the colonies or in the medium?

Correct AnswerverifiedShow Answer

Which type of microscope cannot image live specimens?

Correct AnswerverifiedShow Answer

The procedures for culturing a microorganism require the use of a microscope.

Correct AnswerverifiedShow Answer

The bending of light rays as they pass from one medium to another is called refraction.

Correct AnswerverifiedShow Answer

Which type of microscope bombards a whole, metal-coated specimen with electrons moving back and forth over it?

Correct AnswerverifiedShow Answer

Which of the following is not a benefit of agar as a solid medium?

Correct AnswerverifiedShow Answer

What do the Gram stain, the acid-fast stain, and the endospore stain have in common?

Correct AnswerverifiedShow Answer

Some microbes are not capable of growing on artificial media as pure cultures.

Correct AnswerverifiedShow Answer

Agar is an important component of media because

Correct AnswerverifiedShow Answer

Which term refers to the microscope's ability to show two separate objects as discrete and distinct?

Correct AnswerverifiedShow Answer

The term that refers to the purposeful addition of microorganisms into a laboratory nutrient medium is ______.

Correct AnswerverifiedShow Answer

Which type of microscope achieves the greatest resolution and highest magnification?

Correct AnswerverifiedShow Answer

Which of the following parts of the microscope magnifies the specimen to produce the virtual image of the specimen?

Correct AnswerverifiedShow Answer

A common medium for culturing fastidious bacteria is ______.

Correct AnswerverifiedShow Answer

All of the following are diameters of cells that would be resolved in a microscope with a limit of resolution of 0.2 µm except ______.

Correct AnswerverifiedShow Answer

A microbiologist makes a fixed smear of bacterial cells and stains it with methylene blue.All cells appear blue under the oil immersion lens.This is an example of ______.

Correct AnswerverifiedShow Answer

Which of the following parts of a microscope magnifies the specimen to produce a real image of the specimen?

Correct AnswerverifiedShow Answer

The correct microbiological term for the sample of specimen that is put into a nutrient medium to produce a culture is ______.

Correct AnswerverifiedShow Answer

What is the term for a culture made from one isolated colony?

Correct AnswerverifiedShow Answer

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