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Exam 17: Diagnosing Infections

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Question 21

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You have a very sore throat with symptoms indicating strep throat.Since you are taking microbiology right now,you decide that you will take a swab from your throat and swab it onto an enrichment medium like a blood agar plate or a brain-heart infusion agar plate.Why is this not a good idea? A. Due to the fact that you cannot easily see the sampling site, there is a high probability that you will not be able to obtain your own specimen without contaminating the sample with your own normal biota. B. You are on a diet now, and you are afraid that the food that you are eating will in some way impact the growth of the bacteria. As a result, you will not get valid results. C. You use a mouthwash a couple of times a day, so you think that the antibacterial properties of the mouthwash will inhibit or kill the oral bacteria and results in invalid results. D. All of these statements are correct.

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A.Due to the fact that you cannot easily...

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Question 22

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Nucleic acid testing is appropriate as a testing method when one is not sure what microorganisms are present in the specimen.

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B) False

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Question 23

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All of the following are routinely collected for microbial analysis of a specimen except ______.

Which test is especially good for bacteria that are not readily cultivated in the lab?

Serological tests should have low sensitivity and specificity.

Serological testing always involves reactions between specific antibody and antigen.

A serum titer involves

Mycobacterium tuberculosis has traditionally been identified in patient samples using biochemical tests,and more recently through DNA probe analysis.In vivo testing can also be completed using the tuberculin skin test.A more recent test that seems to be surpassing the others is the QuantiFERON-TB gold test.Blood samples are mixed with protein antigens of M.tuberculosis.After 16 to 24 hours of incubation,the amount of the cytokine interferon produced by the patient's cells is measured.If the patient is infected with M.tuberculosis,their WBCs will release interferon in response to contact with the tuberculosis protein antigens.This test falls under which category of identification tests?

The testing method involving the separation of serum proteins by electrophoresis,their subsequent transfer to special filters,and then probing with patient sera is called

Which of the following is not an agglutination test?

Which test cannot be used for identification of a person's HIV status?

Viruses can be used to identify bacteria because of viral specificity for a host cell.This identification method is called ______.

The radioimmunoassay test is used frequently for

What type of test will detect cell-associated antigens?

The tuberculin skin test is read

What type of test affixes an antigen to an inert particle such as a latex bead?

The tuberculin test is an example of an in vitro serological test.

Which test compares the percentage of cytosine and guanine bases to adenine and thymine bases?

Which test uses radioactive isotopes to label antibodies or antigens in order to detect minute amounts of corresponding antigen or antibody?

A patient's vaginal specimen is sent from the walk-in clinic to the microbiology laboratory.There it is cultured on bacteriological media and then placed into an ambient air incubator set at 22% oxygen and 37 degrees Celsius.The patient is strongly suspected of having gonorrhea.She had sexual contact with a partner recently diagnosed with gonorrhea,and she is now displaying the typical symptoms of a vaginal exudateor fluid.Neisseria gonorrhoeae is a microaerophilic gram-negative coccus-shaped bacterium.Seventy-two hours later,the culture has still not grown Neisseria gonorrhoeae so the lab reports the results back as negative.Interpret this result. A. The inoculating loop was too hot while streaking the specimen onto agar medium, and the bacteria were all killed. B. The clinical personnel took the sample from an incorrect body area. C. The culture was placed into the wrong environment for Neisseria gonorrhoeae. D. Neisseria gonorrhoeae cannot be grown in bacteriological media and requires genetic analysis for identification.