Question 1

The enzyme required to attach the sticky ends of DNA and used when splicing DNA fragments into other DNA is ______.

Accepted Answer

A)   endonuclease
B)   ligase
C)   reverse transcriptase
D)   helicaseE) A) and B)
F) B) and C) B

Question 2

Identification of unique DNA fingerprints relies on the presence of single nucleotide polymorphisms among samples.

Accepted Answer

A) True 
 B)False

Question 3

Each of the following are features of a cloning host except

Accepted Answer

A)   quick generation time.
B)   minimal growth requirements.
C)   mapped genome.
D)   pathogenicity.
E)   transformable.F) A) and D)
G) B) and C)

Question 4

You want to identify Mycobacterium tuberculosis in a patient sputum sample.Which procedure would be most useful in this case?

Accepted Answer

A)   Whole genome sequencing
B)   Polymerase chain reaction
C)   DNA probe analysis
D)   Microarray analysisE) A) and B)
F) All of the above

Question 5

Before the advent of biotechnology,diabetics would use insulin harvested from cows.Now they receive human insulin produced through ______.

Accepted Answer

A)   gel electrophoresis
B)   gene therapy
C)   PCR
D)   recombinant DNA technologyE) B) and C)
F) A) and D)

Question 6

EcoRI and HindIII are ______.

Accepted Answer

A)   palindromes
B)   reverse transcriptases
C)   restriction endonucleases
D)   ligases
E)   DNA polymerasesF) B) and C)
G) C) and D)

Question 7

What type of DNA map gives locations and sizes of DNA sections?

Accepted Answer

A)   Linkage
B)   Sequence
C)   Physical
D)   Geographical
E)   ChromosomalF) A) and B)
G) D) and E)

Question 8

The process of introducing a needed,normal gene into human cells is called DNA mapping.

Accepted Answer

A) True 
 B)False

Question 9

One reason the field of proteomics is continually evolving following the systematic study of an organism's genome is because

Accepted Answer

A)   it became apparent that while the organism's genome is relatively stable, protein expression in an organism is constantly changing.
B)   the expression of proteins is fixed in each organism so sequencing the genome is essential to understanding phenotypes.
C)   it became apparent that protein expression is relatively uniform, whereas the genome is constantly changing.
D)   once the genome had been sequenced, all protein expression was understood.E) B) and C)
F) A) and D)

Question 10

The study of genomes of a particular community is called ______.

Accepted Answer

A)   metabolomics
B)   proteomics
C)   genomology
D)   genomics
E)   metagenomicsF) D) and E)
G) C) and D) E

Question 11

Each of the following are features of a cloning vector except

Accepted Answer

A)   origin of replication.
B)   reverse transcriptases.
C)   genetic markers used to screen for recombinants.
D)   capacity for large inserts.
E)   multiple cloning sites.F) C) and D)
G) C) and E)

Question 12

The commercial product Frostban consists of a genetically altered bacterium which prevents ice crystals from forming on plants,thereby reducing freezing of plants and financial distress to the farmers as a result of freezing weather.This product contains a strain of ______.

Accepted Answer

A)   Escherichia coli
B)   Saccharomyces cerevisiae
C)   Thermus aquaticus
D)   Pseudomonas fluorescens
E)   Pseudomonas syringaeF) B) and D)
G) B) and C)

Question 13

Which of the following is a list of the materials required for PCR?

Accepted Answer

A)   Primers, Taq DNA polymerase, nucleotides
B)   Primers, Taq RNA polymerase, nucleotides
C)   Reverse transcriptase, Taq RNA polymerase, nucleotides
D)   Reverse transcriptase, Taq DNA polymerase, nucleotidesE) B) and D)
F) A) and D)

Question 14

Which step in gene mapping involves transformation?

Accepted Answer

A)   Target DNA removed from cells and isolated
B)   Cloning host receives plasmid
C)   Target DNA and plasmid treated with the same restriction endonuclease
D)   Desired protein is produced by cloning host
E)   Gene is amplified by multiplication of cloning hostF) A) and B)
G) C) and D)

Question 15

DNA polymerases used in PCR

Accepted Answer

A)   use an RNA template to make complementary DNA.
B)   must remain active at very cold temperatures.
C)   include Taq polymerases and Vent polymerase.
D)   are labeled with fluorescent dyes.E) None of the above
F) B) and C) C

Question 16

Any use of an organism's biochemical processes to create a product is referred to as ______.

Accepted Answer

A)   genetic engineering
B)   biotechnology
C)   recombinant DNA
D)   gel electrophoresis
E)   gene probesF) B) and E)
G) None of the above

Question 17

Which of the following is not true of vectors?

Accepted Answer

A)   They are easy to manipulate.
B)   They can detect RNA in cells.
C)   An origin of replication (ORI)  is present.
D)   They contain a gene for drug resistance.
E)   They must accept DNA of desired size.F) B) and C)
G) B) and D)

Question 18

You have made a specific DNA probe that will bind to a key sequence on the DNA of Neisseria gonorrhoeae,allowing your company to market a gonorrhea test kit that can be used to identify the bacterium in genital tract specimens.However,upon testing it out against a known Neisseria gonorrhoeae culture,you find that it does not work.Which of the following is a possible explanation for this negative result?

Accepted Answer

A)   You forgot to label the probe sequence with a reporter molecule.
B)   You forgot to add the mRNA to the DNA probe sample.
C)   You forgot to digest the probe with restriction endonucleases.
D)   You forgot to incubate the test at the 75oC temperature required for hybridization.E) A) and D)
F) A) and B)

Question 19

Fluorescent in situ hybridization (FISH) probes are applied to intact cells and observed microscopically for the presence and location of

Accepted Answer

A)   DNA.
B)   proteins.
C)   a specific genetic marker sequence.
D)   recombinant DNA.
E)   RNA.F) D) and E)
G) A) and C)

Question 20

A technique that separates a readable pattern of DNA fragments is ______.

Accepted Answer

A)   genetic engineering
B)   biotechnology
C)   recombinant DNA
D)   gel electrophoresis
E)   gene probesF) None of the above
G) A) and C)

Exam 10: Genetic Engineering and Recombinant Dna

The enzyme required to attach the sticky ends of DNA and used when splicing DNA fragments into other DNA is ______.

Correct Answer
verified
B

Identification of unique DNA fingerprints relies on the presence of single nucleotide polymorphisms among samples.

Correct Answer
verified

Each of the following are features of a cloning host except

Correct Answer
verified

You want to identify Mycobacterium tuberculosis in a patient sputum sample.Which procedure would be most useful in this case?

Correct Answer
verified

Before the advent of biotechnology,diabetics would use insulin harvested from cows.Now they receive human insulin produced through ______.

Correct Answer
verified

EcoRI and HindIII are ______.

Correct Answer
verified

What type of DNA map gives locations and sizes of DNA sections?

Correct Answer
verified

The process of introducing a needed,normal gene into human cells is called DNA mapping.

Correct Answer
verified

One reason the field of proteomics is continually evolving following the systematic study of an organism's genome is because

Correct Answer
verified

The study of genomes of a particular community is called ______.

Correct Answer
verified
E

Each of the following are features of a cloning vector except

Correct Answer
verified

The commercial product Frostban consists of a genetically altered bacterium which prevents ice crystals from forming on plants,thereby reducing freezing of plants and financial distress to the farmers as a result of freezing weather.This product contains a strain of ______.

Correct Answer
verified

Which of the following is a list of the materials required for PCR?

Correct Answer
verified

Which step in gene mapping involves transformation?

Correct Answer
verified

DNA polymerases used in PCR

Correct Answer
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C

Any use of an organism's biochemical processes to create a product is referred to as ______.

Correct Answer
verified

Which of the following is not true of vectors?

Correct Answer
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Correct Answer
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Fluorescent in situ hybridization (FISH) probes are applied to intact cells and observed microscopically for the presence and location of

Correct Answer
verified

A technique that separates a readable pattern of DNA fragments is ______.

Correct Answer
verified

Exam 10: Genetic Engineering and Recombinant Dna

The enzyme required to attach the sticky ends of DNA and used when splicing DNA fragments into other DNA is ______.

Correct AnswerverifiedB

Identification of unique DNA fingerprints relies on the presence of single nucleotide polymorphisms among samples.

Correct AnswerverifiedShow Answer

Each of the following are features of a cloning host except

Correct AnswerverifiedShow Answer

You want to identify Mycobacterium tuberculosis in a patient sputum sample.Which procedure would be most useful in this case?

Correct AnswerverifiedShow Answer

Before the advent of biotechnology,diabetics would use insulin harvested from cows.Now they receive human insulin produced through ______.

Correct AnswerverifiedShow Answer

EcoRI and HindIII are ______.

Correct AnswerverifiedShow Answer

What type of DNA map gives locations and sizes of DNA sections?

Correct AnswerverifiedShow Answer

The process of introducing a needed,normal gene into human cells is called DNA mapping.

Correct AnswerverifiedShow Answer

One reason the field of proteomics is continually evolving following the systematic study of an organism's genome is because

Correct AnswerverifiedShow Answer

The study of genomes of a particular community is called ______.

Correct AnswerverifiedE

Each of the following are features of a cloning vector except

Correct AnswerverifiedShow Answer

The commercial product Frostban consists of a genetically altered bacterium which prevents ice crystals from forming on plants,thereby reducing freezing of plants and financial distress to the farmers as a result of freezing weather.This product contains a strain of ______.

Correct AnswerverifiedShow Answer

Which of the following is a list of the materials required for PCR?

Correct AnswerverifiedShow Answer

Which step in gene mapping involves transformation?

Correct AnswerverifiedShow Answer

DNA polymerases used in PCR

Correct AnswerverifiedC

Any use of an organism's biochemical processes to create a product is referred to as ______.

Correct AnswerverifiedShow Answer

Which of the following is not true of vectors?

Correct AnswerverifiedShow Answer

Correct AnswerverifiedShow Answer

Fluorescent in situ hybridization (FISH) probes are applied to intact cells and observed microscopically for the presence and location of

Correct AnswerverifiedShow Answer

A technique that separates a readable pattern of DNA fragments is ______.

Correct AnswerverifiedShow Answer

Correct Answer
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B

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E

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C

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