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The process by which a cell uses ATP to enclose a substance in its membrane,by forming a vacuole and engulfing it,is called ______.


A) active transport
B) facilitated diffusion
C) endocytosis
D) diffusion
E) osmosis

F) A) and C)
G) A) and B)

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Anaerobes can be cultured in a CO2 environment.

A) True
B) False

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The methanogens,producers of methane gas,require environments that


A) have abundant oxygen and CO2.
B) are directly exposed to sunlight.
C) are extremely cold.
D) are very acidic.
E) are anaerobic with hydrogen gas and CO2.

F) A) and D)
G) B) and E)

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An organic nutrient that cannot be synthesized by the organism and must be provided is called a(n) ______.


A) element
B) trace element
C) growth factor
D) macronutrient
E) compound

F) B) and D)
G) A) and B)

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A microorganism that has an optimum growth temperature of 37°C,but can survive short exposure to high temperatures is called a(n) ______.


A) thermophile
B) psychrophile
C) thermoduric microbe
D) extremophile
E) facultative psychrophile

F) B) and E)
G) B) and D)

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The toxic superoxide ion is converted to harmless oxygen by two enzymes,


A) superoxide dismutase and hydrogen peroxidase.
B) superoxide dismutase and oxidase.
C) superoxide dismutase and catalase.
D) catalase and hydrogen peroxidase.
E) catalase and oxidase.

F) C) and D)
G) None of the above

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Most microorganisms can only grow in habitats that are similar to human body conditions.

A) True
B) False

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In a commensal relationship,the commensal benefits but the cohabitant is neither harmed nor benefited.

A) True
B) False

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Inorganic nitrogen must be converted to ammonia to be used by a cell.

A) True
B) False

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A closed culture system is used to determine a population growth curve.

A) True
B) False

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The movement of substances from higher to lower concentration across a semipermeable membrane via a specific protein carrier but without energy expenditure is called ______.


A) facilitated diffusion
B) active transport
C) diffusion
D) endocytosis
E) osmosis

F) A) and D)
G) C) and D)

Correct Answer

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As described in "A Note About Bacterial Reproduction -- and the "Culture Bias,"" the organism Epulopiscium does not divide by binary fission.Rather,each cell increases in size and divides to produce multiple daughter cells that are held within the original cell well.After lysis,those daughters are released to repeat the process. Assuming Epulopiscium could be grown in pure culture in the laboratory in broth and on solid media (it currently cannot) ,which method would be best for measuring the increase in biomass during growth?


A) The viable plate count; each colony derives from a single cell and the number of colonies equals the number of cells.
B) Turbidity readings from a spectrophotometer; the increase in biomass will directly vary with the turbidity of the culture.
C) Chemostat growth; it will prevent the culture from entering the death phase.
D) Direct microscopic count; the experimenter can directly count the number of cells and extrapolate to the biomass.

E) A) and B)
F) A) and C)

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An organism that uses CO2 for its carbon needs and sunlight for its energy needs would be called a ______.


A) chemoheterotroph
B) halotroph
C) saprobe
D) photoautotroph
E) heterotroph

F) C) and D)
G) B) and D)

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In the viable plate count method,a measured volume of a culture is evenly spread across an agar surface and incubated.Each _____ represents one _____ from the original sample.


A) cell; colony
B) colony; cell
C) generation; cell
D) species; colony
E) cell; cell

F) A) and B)
G) A) and C)

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Which compound has the highest concentration in a cell?


A) NH3
B) CO2
C) H2O
D) CH4
E) Glucose

F) A) and D)
G) C) and D)

Correct Answer

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E.coli bacteria normally live in the human gut and produce vitamin K that the body uses.This is best termed a ______ relationship.


A) mutualistic
B) saprobic
C) parasitic
D) antagonistic

E) None of the above
F) B) and C)

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When it is important to count the number of cells,determine cell size,and differentiate between dead and live cells,a(n) ______ is used.


A) SEM
B) Coulter counter
C) methylene indicator dye
D) flow cytometer
E) spectrophotometer

F) A) and E)
G) B) and E)

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