Question 1

Which of the following techniques is mismatched with its use in DNA fingerprinting?

Accepted Answer

A)  restriction endonucleases to cut DNA
B)  PCR amplification to get more copies of DNA
C)  electrophoresis to separate DNA fragments
D)  hybridization probes to digest the DNA sample
E)  Southern blot for a visual record of DNA fragmentsF) D) and E)
G) A) and B) D

Question 2

DNA polymerases used in PCR

Accepted Answer

A)  use an RNA template to make complementary DNA.
B)  must remain active at very cold temperatures.
C)  include Taq polymerases and Vent polymerase.
D)  are labeled with fluorescent dyes.E) C) and D)
F) None of the above

Question 3

Which PCR step synthesizes complimentary DNA strands?

Accepted Answer

A)  add DNA polymerase and nucleotides at 72 ∘\circ∘  C
B)  cool DNA to between 50 ∘\circ∘  C and 65 ∘\circ∘ C
C)  add primers
D)  heat target DNA to 94 ∘\circ∘  C
E)  repeat the cycle of heating and cooling F) None of the above
G) C) and D)

Question 4

E.coli is an excellent host for cloning because it possesses the mechanisms for processing and modifying proteins.

Accepted Answer

A) True 
 B)False

Question 5

The various techniques by which scientists manipulate DNA in the lab are termed

Accepted Answer

A)  genetic engineering.
B)  biotechnology.
C)  recombinant DNA.
D)  gel electrophoresis.
E)  gene probes.F) B) and E)
G) A) and B)

Question 6

This is the second step in gene mapping

Accepted Answer

A)  target DNA removed from cells and isolated.
B)  cloning host treated with calcium chloride and receives plasmid.
C)  separate DNA fragments with gel electrophoresis.
D)  desired protein is produced by cloning host.
E)  gene is amplified by multiplication of cloning host.F) A) and C)
G) A) and E)

Question 7

Which of the following is not true of fluorescent in situ hybridization (FISH) ?

Accepted Answer

A)  applied to intact cells
B)  can locate genes on chromosomes
C)  can identify unknown bacteria without culturing
D)  uses electrophoresis to separate the DNA
E)  can detect RNA in cellsF) A) and E)
G) A) and D)

Question 8

A technique that separates a readable pattern of DNA fragments is

Accepted Answer

A)  genetic engineering.
B)  biotechnology.
C)  recombinant DNA.
D)  gel electrophoresis.
E)  gene probes.F) None of the above
G) A) and B)

Question 9

When DNA is heated,the two strands will separate.

Accepted Answer

A) True 
 B)False

Question 10

EcoRI and HindIII are

Accepted Answer

A)  palindromes.
B)  reverse transcriptases.
C)  restriction endonucleases.
D)  ligases.
E)  DNA polymerases.F) A) and E)
G) B) and E)

Question 11

Transformation and transduction are methods used to introduce DNA into host cells.

Accepted Answer

A) True 
 B)False

Question 12

The first genetically engineered protein approved for human use was

Accepted Answer

A)  human growth hormone.
B)  hemophilia factor VIII.
C)  human testosterone.
D)  human insulin.
E)  human adrenaline.F) C) and D)
G) All of the above

Question 13

This is a promising treatment for stopping the expression of an unwanted gene:

Accepted Answer

A)  cloning
B)  gene therapy
C)  antisense therapy
D)  DNA fingerprintingE) A) and B)
F) None of the above

Question 14

Restriction endonucleases recognize and clip DNA base sequences called

Accepted Answer

A)  codons.
B)  palindromes.
C)  introns.
D)  exons.
E)  genes.F) C) and E)
G) C) and D) B

Question 15

Restriction endonucleases are obtained from various species of bacteria.

Accepted Answer

A) True 
 B)False

Question 16

If you start with 3 double-stranded DNA fragments,after 4 cycles of PCR you will have ____ fragments.

Accepted Answer

A)  12
B)  24
C)  27
D)  48
E)  81F) B) and C)
G) A) and E)

Question 17

In choosing the vector used to carry human genes into a host cell,which of the following should be the important consideration?

Accepted Answer

A)  whether the vector contains RNA or DNA
B)  what kind of plasmid it is
C)  whether the vector comes from a bacterium or a virus
D)  how much donor DNA can be carried on the vectorE) A) and C)
F) A) and B)

Question 18

Analysis of DNA fragments in gel electrophoresis is based on

Accepted Answer

A)  larger fragments moving slowly and remaining closer to the wells.
B)  DNA having an overall negative charge and moving to the positive pole.
C)  DNA fragments being stained so that they can be seen.
D)  application of an electric current through the gel causing DNA fragments to migrate.
E)  All of the choices are correct.F) A) and B)
G) D) and E)

Question 19

The enzyme required to attach the sticky ends of DNA and used when splicing DNA fragments into other DNA is:

Accepted Answer

A)  helicase.
B)  ligase.
C)  reverse transcriptase.
D)  endonuclease.E) A) and D)
F) A) and C) B

Question 20

It is possible to identify mRNA molecules using fluorescently labeled cDNA.

Accepted Answer

A) True 
 B)False

Exam 10: Genetic Engineering and Recombinant Dna

Which of the following techniques is mismatched with its use in DNA fingerprinting?

Correct AnswerverifiedD

DNA polymerases used in PCR

Correct AnswerverifiedShow Answer

Which PCR step synthesizes complimentary DNA strands?

Correct AnswerverifiedShow Answer

E.coli is an excellent host for cloning because it possesses the mechanisms for processing and modifying proteins.

Correct AnswerverifiedShow Answer

The various techniques by which scientists manipulate DNA in the lab are termed

Correct AnswerverifiedShow Answer

This is the second step in gene mapping

Correct AnswerverifiedShow Answer

Which of the following is not true of fluorescent in situ hybridization (FISH) ?

Correct AnswerverifiedShow Answer

A technique that separates a readable pattern of DNA fragments is

Correct AnswerverifiedShow Answer

When DNA is heated,the two strands will separate.

Correct AnswerverifiedShow Answer

EcoRI and HindIII are

Correct AnswerverifiedShow Answer

Transformation and transduction are methods used to introduce DNA into host cells.

Correct AnswerverifiedShow Answer

The first genetically engineered protein approved for human use was

Correct AnswerverifiedShow Answer

This is a promising treatment for stopping the expression of an unwanted gene:

Correct AnswerverifiedShow Answer

Restriction endonucleases recognize and clip DNA base sequences called

Correct AnswerverifiedB

Restriction endonucleases are obtained from various species of bacteria.

Correct AnswerverifiedShow Answer

If you start with 3 double-stranded DNA fragments,after 4 cycles of PCR you will have ____ fragments.

Correct AnswerverifiedShow Answer

In choosing the vector used to carry human genes into a host cell,which of the following should be the important consideration?

Correct AnswerverifiedShow Answer

Analysis of DNA fragments in gel electrophoresis is based on

Correct AnswerverifiedShow Answer

The enzyme required to attach the sticky ends of DNA and used when splicing DNA fragments into other DNA is:

Correct AnswerverifiedB

It is possible to identify mRNA molecules using fluorescently labeled cDNA.

Correct AnswerverifiedShow Answer

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D

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B

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B

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