Question 1

Which step can occur even more rapidly by PCR?

Accepted Answer

A)  target DNA removed from cells and isolated
B)  cloning host treated with calcium chloride and receives plasmid
C)  target DNA and plasmid treated with the same restriction endonuclease
D)  desired protein is produced by cloning host
E)  gene is amplified by multiplication of cloning hostF) A) and C)
G) A) and E)

Question 2

In the formation of recombinant DNA,what enzyme is needed to seal the sticky ends of genes into plasmids or chromosomes?

Accepted Answer

A)  DNA polymerase I
B)  DNA polymerase II
C)  DNA helicase
D)  DNA ligase
E)  primaseF) A) and C)
G) B) and C)

Question 3

Which PCR step causes the denaturation of double-stranded DNA?

Accepted Answer

A)  add DNA polymerase and nucleotides at 72 ∘\circ∘  C
B)  cool DNA to between 50 ∘\circ∘  C and 65 ∘\circ∘  C
C)  add primers
D)  heat target DNA to 94 ∘\circ∘  C
E)  repeat the cycle of heating and cooling F) A) and B)
G) A) and C)

Question 4

Thermococcus litoralis and Thermus aquaticus are thermophilic bacteria that are

Accepted Answer

A)  used as cloning vectors.
B)  sources of heat-stable DNA polymerases.
C)  genetically engineered bacteria.
D)  principal sources of restriction endonucleases.E) All of the above
F) None of the above

Question 5

Amplification of DNA is accomplished by

Accepted Answer

A)  polymerase chain reaction.
B)  DNA sequencing.
C)  gene probes.
D)  Southern blot.
E)  Western blot.F) C) and D)
G) A) and B)

Question 6

Each of the following are features of a vector except

Accepted Answer

A)  origin of replication.
B)  reverse transcriptases.
C)  genetic markers used to screen for recombinants
D)  capacity for large inserts.
E)  multiple cloning sites.F) D) and E)
G) All of the above

Question 7

Viruses are often used as cloning hosts in recombinant DNA methods.

Accepted Answer

A) True 
 B)False

Question 8

Which step involves transformation?

Accepted Answer

A)  target DNA removed from cells and isolated
B)  cloning host receives plasmid
C)  target DNA and plasmid treated with the same restriction endonuclease
D)  desired protein is produced by cloning host
E)  gene is amplified by multiplication of cloning hostF) C) and D)
G) None of the above

Question 9

The commercial product Frostban consists of a genetically altered bacterium which prevents ice crystals from forming on plants,thereby reducing freezing of plants and financial distress to the farmers as a result of freezing weather.This product contains a strain of

Accepted Answer

A)  Escherichia coli.
B)  Saccharomyces cerevisiae.
C)  Thermus aquaticus.
D)  Pseudomonas syringae.
E)  Pseudomonas fluorescens.F) B) and D)
G) A) and E)

Question 10

After three replication cycles in PCR,there will be a total of three double-stranded DNA molecules.

Accepted Answer

A) True 
 B)False

Question 11

Why is an enzyme from a thermophilic bacterium used in PCR?

Accepted Answer

A)  The enzyme makes DNA that is more similar to human DNA.
B)  It is cheaper to obtain from live microorganisms than producing the enzyme in a lab.
C)  This thermohile's enzyme will synthesize DNA.
D)  DNA is replicated at a high temperature that denatures most proteins.E) C) and D)
F) A) and D)

Question 12

Gene probes can be labeled for detection with reporter molecules such as

Accepted Answer

A)  enzymes.
B)  fluorescent dyes.
C)  radioisotopes.
D)  All of the choices above can be used.E) All of the above
F) None of the above

Question 13

It is now possible to very quickly map the genome of an organism or virus.

Accepted Answer

A) True 
 B)False

Question 14

Fluorescent in situ hybridization (FISH) probes are applied to intact cells and observed microscopically for the presence and location of

Accepted Answer

A)  DNA.
B)  RNA.
C)  proteins.
D)  recombinant DNA.
E)  specific genetic marker sequence on genes.F) All of the above
G) None of the above

Question 15

The deliberate removal of genetic material from one organism and its subsequent transfer into the genome of another organism is a specific technique called

Accepted Answer

A)  genetic engineering.
B)  biotechnology.
C)  recombinant DNA technology.
D)  gel electrophoresis.
E)  gene probe technology.F) A) and D)
G) A) and B)

Question 16

Each of the following are features of a cloning host except

Accepted Answer

A)  quick generation time.
B)  minimal growth requirements.
C)  mapped genome.
D)  pathogenic.
E)  transformable.F) B) and C)
G) A) and E)

Question 17

Which of the following it a list of the materials required for PCR?

Accepted Answer

A)  reverse transcriptase,Taq RNA polymerase,nucleotides
B)  reverse transcriptase,Taq DNA polymerase,nucleotides
C)  primers,Taq DNA polymerase,nucleotides
D)  primers,Taq RNA polymerase,nucleotidesE) A) and B)
F) A) and D)

Question 18

Transgenic animals are referred to as _____ modified organisms.

Accepted Answer

A)  genetically
B)  naturally
C)  chemically
D)  physicallyE) C) and D)
F) A) and B)

Question 19

Reverse transcriptase is used to make cDNA from an RNA template.

Accepted Answer

A) True 
 B)False

Question 20

The size of DNA is often given in the number of _____ that it contains.

Accepted Answer

A)  genes
B)  codons
C)  base pairs
D)  proteins
E)  tripletsF) D) and E)
G) C) and D)

Exam 10: Genetic Engineering and Recombinant Dna

Reverse transcriptase is used to make cDNA from an RNA template.

Correct AnswerverifiedShow Answer

Which step involves transformation?

Correct AnswerverifiedShow Answer

Gene probes can be labeled for detection with reporter molecules such as

Correct AnswerverifiedShow Answer

The deliberate removal of genetic material from one organism and its subsequent transfer into the genome of another organism is a specific technique called

Correct AnswerverifiedShow Answer

Thermococcus litoralis and Thermus aquaticus are thermophilic bacteria that are

Correct AnswerverifiedShow Answer

The size of DNA is often given in the number of _____ that it contains.

Correct AnswerverifiedShow Answer

Each of the following are features of a cloning host except

Correct AnswerverifiedShow Answer

Fluorescent in situ hybridization (FISH) probes are applied to intact cells and observed microscopically for the presence and location of

Correct AnswerverifiedShow Answer

Which step can occur even more rapidly by PCR?

Correct AnswerverifiedShow Answer

It is now possible to very quickly map the genome of an organism or virus.

Correct AnswerverifiedShow Answer

Why is an enzyme from a thermophilic bacterium used in PCR?

Correct AnswerverifiedShow Answer

Each of the following are features of a vector except

Correct AnswerverifiedShow Answer

Which PCR step causes the denaturation of double-stranded DNA?

Correct AnswerverifiedShow Answer

Amplification of DNA is accomplished by

Correct AnswerverifiedShow Answer

In the formation of recombinant DNA,what enzyme is needed to seal the sticky ends of genes into plasmids or chromosomes?

Correct AnswerverifiedShow Answer

After three replication cycles in PCR,there will be a total of three double-stranded DNA molecules.

Correct AnswerverifiedShow Answer

Viruses are often used as cloning hosts in recombinant DNA methods.

Correct AnswerverifiedShow Answer

Transgenic animals are referred to as _____ modified organisms.

Correct AnswerverifiedShow Answer

Which of the following it a list of the materials required for PCR?

Correct AnswerverifiedShow Answer

The commercial product Frostban consists of a genetically altered bacterium which prevents ice crystals from forming on plants,thereby reducing freezing of plants and financial distress to the farmers as a result of freezing weather.This product contains a strain of

Correct AnswerverifiedShow Answer

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