Question 1

An antibody_____ provides a measure of the quantity of antibodies in the serum.

Accepted Answer

The answer of An antibody_____ provides a measure of the...

Question 2

The more a sample can be diluted and yet still react with antigen, the ______ the concentration of antibodies in that sample and the _______ is its titer.

Accepted Answer

A)  lower; lower
B)  higher; lower
C)  lower; higher
D)  higher; higherE) A) and B)
F) C) and D)

Question 3

In a __________, antigens or antibodies are labeled with radioactive isotopes and traced.

Accepted Answer

The answer of In a __________, antigens or antibodies are...

Question 4

All of the following are methods to diagnose viral infections, except

Accepted Answer

A)  detection of viral nucleic acid using specific probes.
B)  Western Blot.
C)  cells taken from patient are examined for evidence of viral infection.
D)  signs and symptoms.
E)  the light microscope.F) C) and D)
G) C) and E)

Question 5

ELISA tests use a fluorescent dye as a label to trace antigen-antibody reactions.

Accepted Answer

A) True 
 B)False

Question 6

Tests that take place in the lab, such as in a test tube or Petri-dish, outside of a living host, are referred to as _______________ whereas tests that take place in a living host, such as in a patient, are described as ______________.

Accepted Answer

A)  In loco; In vitro
B)  In vitro; In loco
C)  In vitro; In vivo
D)  In loco; In vivoE) All of the above
F) A) and C) C

Question 7

Which of the following choices are true about the dyes fluorescein and rhodamine?

Accepted Answer

A)  They can be used to label antibodies in immunofluorescence tests.
B)  They emit visible light in response to ultraviolet radiation.
C)  They are observed in the fluorescent microscope.
D)  They are used to identify pathogens of chlamydiosis, Legionnaires'disease, and others.
E)  All of these choices are correct.F) A) and D)
G) A) and E)

Question 8

All of the following are correct about the indirect ELISA, except

Accepted Answer

A)  it can detect antibodies in a serum sample.
B)  it is the common screening test for antibodies to Helicobacter.
C)  color development indicates that antibody was not present in the patient's serum.
D)  it is the common screening test for antibodies to Hepatitis A.
E)  a known antigen is adsorbed to the surface of a well.F) B) and D)
G) A) and E)

Question 9

If trying to isolate a specific pathogen from amongst a variety of normal microbiota, which type of media would be most helpful?

Accepted Answer

A)  nutrient agar
B)  differential media
C)  blood agar
D)  selective and differential agarE) None of the above
F) A) and B)

Question 10

In _________ tests, antibody cross-links whole-cell antigens, forming complexes that settle out and form visible clumps in the test chamber.

Accepted Answer

The answer of In _________ tests, antibody cross-links whole-cell antigens,...

Question 11

The indirect ELISA test detects ______ in a patient's blood.

Accepted Answer

A)  antigen
B)  antibody
C)  microorganisms
D)  IgE only
E)  complementF) All of the above
G) A) and B)

Question 12

Mary had an accidental needlestick one night during her shift as an emergency medical technician.Initially, she tested positive for HIV by the ELISA test.Which test would be done next to confirm an HIV infection?

Accepted Answer

A)  immunoelectrophoresis
B)  Ouchterlony
C)  complement fixation
D)  Western Blot
E)  radioimmunoassayF) A) and B)
G) B) and E)

Question 13

Biochemical testing used for identification of pathogens involves each of the following, except

Accepted Answer

A)  inoculation of additional media, incubation, and observation for a color change.
B)  quick tests that take just a few seconds.
C)  examining organisms through the microscope.D) A) and B)
E) A) and C)

Question 14

Some diseases are diagnosed without the need to identify microbes from a patient specimen.

Accepted Answer

A) True 
 B)False

Question 15

Which technique used to diagnose a disease is also called the double diffusion method?

Accepted Answer

A)  Ouchterlony test
B)  VDRL test
C)  immunoelectrophoresis
D)  Western Blot
E)  ELISAF) C) and E)
G) A) and E)

Question 16

A rising antibody titer a few days apart indicates

Accepted Answer

A)  the patient had a past case of the disease.
B)  the patient has a current infection.
C)  nothing, people always have antibodies in their serum.
D)  All of these choices are correct.E) All of the above
F) B) and D) B

Question 17

Pulsed-field gel electrophoresis (PFGE)  is often used to determine whether bacterial isolates from different cases of food-borne illness are the same and can be traced to the same source.This is an example of

Accepted Answer

A)  an immunologic technique.
B)  a genetic method.
C)  a phenotypic method.
D)  a microscopic technique.E) None of the above
F) All of the above B

Question 18

When collecting __________, a "clean catch" method is often used.

Accepted Answer

The answer of When collecting __________, a "clean catch" method...

Question 19

Serological tests should have low sensitivity and specificity.

Accepted Answer

A) True 
 B)False

Question 20

Which of the following can be used for the cultivation of viruses?

Accepted Answer

A)  Enriched broth media
B)  Viral host cells grown in vitro
C)  Enriched agar media
D)  All of these choices.E) All of the above
F) A) and D)

Exam 17: Procedures for Identifying Pathogens and Diagnosing Infections

An antibody_____ provides a measure of the quantity of antibodies in the serum.

Correct AnswerverifiedShow Answer

The more a sample can be diluted and yet still react with antigen, the ______ the concentration of antibodies in that sample and the _______ is its titer.

Correct AnswerverifiedShow Answer

In a __________, antigens or antibodies are labeled with radioactive isotopes and traced.

Correct AnswerverifiedShow Answer

All of the following are methods to diagnose viral infections, except

Correct AnswerverifiedShow Answer

ELISA tests use a fluorescent dye as a label to trace antigen-antibody reactions.

Correct AnswerverifiedShow Answer

Tests that take place in the lab, such as in a test tube or Petri-dish, outside of a living host, are referred to as _______________ whereas tests that take place in a living host, such as in a patient, are described as ______________.

Correct AnswerverifiedC

Which of the following choices are true about the dyes fluorescein and rhodamine?

Correct AnswerverifiedShow Answer

All of the following are correct about the indirect ELISA, except

Correct AnswerverifiedShow Answer

If trying to isolate a specific pathogen from amongst a variety of normal microbiota, which type of media would be most helpful?

Correct AnswerverifiedShow Answer

In _________ tests, antibody cross-links whole-cell antigens, forming complexes that settle out and form visible clumps in the test chamber.

Correct AnswerverifiedShow Answer

The indirect ELISA test detects ______ in a patient's blood.

Correct AnswerverifiedShow Answer

Mary had an accidental needlestick one night during her shift as an emergency medical technician.Initially, she tested positive for HIV by the ELISA test.Which test would be done next to confirm an HIV infection?

Correct AnswerverifiedShow Answer

Biochemical testing used for identification of pathogens involves each of the following, except

Correct AnswerverifiedShow Answer

Some diseases are diagnosed without the need to identify microbes from a patient specimen.

Correct AnswerverifiedShow Answer

Which technique used to diagnose a disease is also called the double diffusion method?

Correct AnswerverifiedShow Answer

A rising antibody titer a few days apart indicates

Correct AnswerverifiedB

Pulsed-field gel electrophoresis (PFGE) is often used to determine whether bacterial isolates from different cases of food-borne illness are the same and can be traced to the same source.This is an example of

Correct AnswerverifiedB

When collecting __________, a "clean catch" method is often used.

Correct AnswerverifiedShow Answer

Serological tests should have low sensitivity and specificity.

Correct AnswerverifiedShow Answer

Which of the following can be used for the cultivation of viruses?

Correct AnswerverifiedShow Answer

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The more a sample can be diluted and yet still react with antigen, the the concentration of antibodies in that sample and the _ is its titer.

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Tests that take place in the lab, such as in a test tube or Petri-dish, outside of a living host, are referred to as _ whereas tests that take place in a living host, such as in a patient, are described as .

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C

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B

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B

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