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_____ is the term for a starting a new culture from an existing one.

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Observing and characterizing colonial growth for size, shape, edge, elevation, color, odor, and texture is part of ___.


A) specimen collection
B) inoculation
C) incubation
D) isolation
E) inspection

F) C) and D)
G) A) and B)

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A medium that is gel-like has less agar in it compared to a solid medium.

A) True
B) False

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All of the following are correct about agar except


A) it is flexible.
B) it melts at the boiling point of water (100°C) .
C) it is a source of nutrition for bacteria.
D) it solidifies below 42°C.
E) it is solid at room temperature.

F) B) and E)
G) B) and D)

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The procedure for culturing a microorganism requires the use of a microscope.

A) True
B) False

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Brain-heart infusion, trypticase soy agar (TSA) , and nutrient agar are all examples of which type of media?


A) synthetic
B) reducing
C) enriched
D) complex
E) selective

F) A) and B)
G) None of the above

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A bacterial cell that is deposited on the surface of an appropriate solid growth media will divide repeatedly until it forms a visible accumulation of bacterial growth.This is known as a bacterial


A) colony.
B) community.
C) biofilm.
D) smear.

E) None of the above
F) All of the above

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All of the following pertain to the fluorescence microscope except it


A) uses electrons to produce a specimen image.
B) is a type of compound microscope.
C) requires the use of dyes like acridine and fluorescein.
D) is commonly used to diagnose certain infections.
E) requires an ultraviolet radiation source.

F) C) and E)
G) B) and E)

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The type of microscope in which you would see brightly illuminated specimens against a black background is


A) bright-field.
B) dark-field.
C) phase-contrast.
D) fluorescence.
E) electron.

F) B) and D)
G) A) and E)

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Which microscope achieves the greatest resolution and highest magnification?


A) bright-field
B) dark-field
C) phase-contrast
D) fluorescence
E) electron

F) C) and E)
G) A) and E)

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All of the following are criteria that are used to classify different types of microbiological growth media EXCEPT


A) physical state or consistency.
B) purpose or function.
C) chemical composition.
D) sensory characteristics (color, smell) .

E) All of the above
F) B) and D)

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All of the following are examples of basic dyes except


A) methylene blue.
B) nigrosin.
C) crystal violet.
D) safranin.
E) carbol fuchsin.

F) A) and B)
G) A) and C)

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Some microbes are not capable of growing on artificial media.

A) True
B) False

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Mixed cultures are also referred to as contaminated cultures.

A) True
B) False

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The primary purpose of staining cells on a microscope slide is to


A) kill them.
B) secure them to the slide.
C) enlarge the cells.
D) add contrast in order to see them better.
E) see motility.

F) A) and E)
G) None of the above

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Which microscope shows cells against a bright background and the intracellular structures of unstained cells based on their varying densities?


A) bright-field
B) dark-field
C) phase-contrast
D) fluorescence
E) electron

F) B) and E)
G) B) and D)

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Basic dyes are


A) attracted to the acidic substances of bacterial cells.
B) anionic.
C) used in negative staining.
D) repelled by cells.
E) dyes such as India ink and nigrosin.

F) A) and E)
G) None of the above

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Which microscope does not use light in forming the specimen image?


A) bright-field
B) dark-field
C) phase-contrast
D) fluorescence
E) electron

F) A) and E)
G) A) and D)

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Magnification is achieved in a compound microscope through the initial magnification of the specimen by the_____ lens.This image is then projected to the _____ lens that will further magnify the specimen to form a virtual image received by the eye.

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Explain how and why immersion oil increases resolution but not magnification when using the 100X objective.

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Answered by ExamLex AI

Immersion oil increases resolution but n...

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