Question 1

A selective medium contains one or more substances that inhibit growth of certain microbes in order to facilitate the growth of other microbes.

Accepted Answer

A) True 
 B)False

Question 2

Which of the following media is useful for cultivating fungi?

Accepted Answer

A)  Sabouraud's agar
B)  MacConkey agar
C)  Tomato juice agar
D)  Phenylethanol agar
E)  Mueller telluriteF) C) and D)
G) A) and B)

Question 3

A common medium used for growing fastidious bacteria is

Accepted Answer

A)  blood agar.
B)  trypticase soy agar.
C)  mannitol salt agar.
D)  MacConkey medium.
E)  a reducing medium.F) B) and E)
G) A) and E)

Question 4

A reducing medium contains

Accepted Answer

A)  sugars that can be fermented.
B)  extra oxygen.
C)  hemoglobin, vitamins, or other growth factors.
D)  substances that remove oxygen.
E)  inhibiting agents.F) A) and E)
G) A) and D)

Question 5

Basic dyes are

Accepted Answer

A)  attracted to the acidic substances of bacterial cells.
B)  anionic.
C)  used in negative staining.
D)  repelled by cells.
E)  dyes such as India ink and nigrosin.F) B) and C)
G) A) and B)

Question 6

Which microscope does not use light in forming the specimen image?

Accepted Answer

A)  bright-field
B)  dark-field
C)  phase-contrast
D)  fluorescence
E)  electronF) C) and D)
G) A) and C)

Question 7

Which microscope achieves the greatest resolution and highest magnification?

Accepted Answer

A)  bright-field
B)  dark-field
C)  phase-contrast
D)  fluorescence
E)  electronF) C) and D)
G) C) and E)

Question 8

Which of the following is essential for development of discrete, isolated colonies?

Accepted Answer

A)  broth medium
B)  differential medium
C)  selective medium
D)  solid medium
E)  assay mediumF) C) and D)
G) A) and C)

Question 9

Some microbes are not capable of growing on artificial media.

Accepted Answer

A) True 
 B)False

Question 10

The primary purpose of staining cells on a microscope slide is to

Accepted Answer

A)  kill them.
B)  secure them to the slide.
C)  enlarge the cells.
D)  add contrast in order to see them better.
E)  see motility.F) A) and E)
G) A) and D)

Question 11

All of the following are examples of basic dyes except

Accepted Answer

A)  Methylene Blue.
B)  Nigrosin.
C)  Crystal Violet.
D)  Safranin.
E)  Carbol Fuchsin.F) B) and D)
G) None of the above

Question 12

Bacteria that require special growth factors and complex organic substances are called

Accepted Answer

A)  fastidious.
B)  pathogenic.
C)  harmless.
D)  anaerobic.
E)  aerobic.F) C) and D)
G) A) and B)

Question 13

Which type of media can be used to determine if a bacteria is motile?

Accepted Answer

A)  SIM
B)  MacConkey
C)  Enriched media
D)  Thayer-Martin media
E)  Chocolate agarF) B) and C)
G) A) and B)

Question 14

Which microscope is the most widely used to show stained cells against a bright background?

Accepted Answer

A)  bright-field
B)  dark-field
C)  phase-contrast
D)  fluorescence
E)  electronF) A) and E)
G) A) and B)

Question 15

If a microbiologist is studying a specimen at a total magnification of 950X, what is the magnifying power of the objective lens if the ocular lens is 10X?

Accepted Answer

A)  100X
B)  950X
C)  85X
D)  850X
E)  95XF) C) and E)
G) A) and E)

Question 16

Explain the difference and significance between a contaminated culture and a mixed culture.

Accepted Answer

A contaminated culture refers to a cultu...

Question 17

A media is designed that allows only staphylococci to grow.In addition, S.aureus colonies have a yellow halo around them and other staphylococci appear white.This type of media is:

Accepted Answer

A)  selective only.
B)  differential only.
C)  both selective and differential.
D)  a reducing media.
E)  enriched.F) A) and C)
G) All of the above

Question 18

All of the following are correct about agar except

Accepted Answer

A)  it is flexible.
B)  it melts at the boiling point of water (100 ∘\circ∘ 
C)  .
C)  it is a source of nutrition for bacteria.
D)  it solidifies below 42 ∘\circ∘ C.
E)  it is solid at room temperature. F) A) and C)
G) A) and B)

Question 19

Which of the following magnifies the specimen to produce the real image of the specimen?

Accepted Answer

A)  condenser
B)  objective lens
C)  ocular lens
D)  body
E)  nosepieceF) None of the above
G) A) and B)

Question 20

Magnification is achieved in a compound microscope through the initial magnification of the specimen by the _____ lens.This image is then projected to the _____ lens that will further magnify the specimen to form a virtual image received by the eye.

Accepted Answer

The answer of Magnification is achieved in a compound microscope...

Exam 3: Tools of the Laboratory: Methods of Studying Microorganisms

All of the following are examples of basic dyes except

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A common medium used for growing fastidious bacteria is

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Basic dyes are

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All of the following are correct about agar except

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Which microscope does not use light in forming the specimen image?

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A reducing medium contains

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Which of the following is essential for development of discrete, isolated colonies?

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A media is designed that allows only staphylococci to grow.In addition, S.aureus colonies have a yellow halo around them and other staphylococci appear white.This type of media is:

Correct AnswerverifiedShow Answer

Magnification is achieved in a compound microscope through the initial magnification of the specimen by the _____ lens.This image is then projected to the _____ lens that will further magnify the specimen to form a virtual image received by the eye.

Correct AnswerverifiedShow Answer

The primary purpose of staining cells on a microscope slide is to

Correct AnswerverifiedShow Answer

If a microbiologist is studying a specimen at a total magnification of 950X, what is the magnifying power of the objective lens if the ocular lens is 10X?

Correct AnswerverifiedShow Answer

Which of the following magnifies the specimen to produce the real image of the specimen?

Correct AnswerverifiedShow Answer

Bacteria that require special growth factors and complex organic substances are called

Correct AnswerverifiedShow Answer

Which of the following media is useful for cultivating fungi?

Correct AnswerverifiedShow Answer

A selective medium contains one or more substances that inhibit growth of certain microbes in order to facilitate the growth of other microbes.

Correct AnswerverifiedShow Answer

Which microscope is the most widely used to show stained cells against a bright background?

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Which type of media can be used to determine if a bacteria is motile?

Correct AnswerverifiedShow Answer

Explain the difference and significance between a contaminated culture and a mixed culture.

Correct AnswerAnswered by ExamLex AIA contaminated culture refers to a cultu...View AnswerShow Answer

Some microbes are not capable of growing on artificial media.

Correct AnswerverifiedShow Answer

Which microscope achieves the greatest resolution and highest magnification?

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Magnification is achieved in a compound microscope through the initial magnification of the specimen by the _ lens.This image is then projected to the _ lens that will further magnify the specimen to form a virtual image received by the eye.

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A contaminated culture refers to a cultu...
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