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A major difference in the production of transgenic bacteria,plants,and animals is that


A) Transgenic bacteria do not pass on their genetic modification to offspring while plants and animals do.
B) Transgenic animals require the use of host animals to carry the genetically modified embryo while bacteria and plants do not require this.
C) Transgenic bacteria and plants receive genes from other species of organisms while animals are only able to incorporate genes from their own species.
D) Transgenic animals are the most useful of the GMOs while transgenic plants and bacteria are only created to perfect the techniques.
E) Transgenic bacteria, animals, and plants can only incorporate genes from their own species so the process for each species differs from the process for any other species.

F) B) and D)
G) A) and C)

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B

Which of the following is a site of ex vivo gene therapy?


A) bone marrow
B) muscle
C) blood
D) lungs
E) brain

F) A) and D)
G) C) and D)

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A method used to determine if the DNA found at a crime scene belongs to a particular individual is short tandem repeat (STR) .This method employs


A) Repeated sequences found at multiple sites in the DNA.
B) One particular gene found in the individual in question.
C) Fluorescent labeling of DNA found in the mitochondria.
D) Repeated sequences found at one specific site in the DNA.
E) The repeated segments at the end of chromosomes called telomeres.

F) B) and E)
G) A) and B)

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Which of the following terms applies to the study of all of the proteins produced by an organism?


A) genomics
B) bioinformatics
C) proteomics
D) genetic profiling
E) genetics

F) A) and B)
G) A) and E)

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C

Which species has an estimated 6,300 genes in it's genome?


A) yeast
B) roundworms
C) human beings
D) fruit fly
E) mouse

F) None of the above
G) B) and E)

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Enzymes from the thermophile organism Thermus aquaticus has allowed the development of which of the following procedures?


A) Genetic engineering
B) Recombinant DNA
C) DNA cloning
D) polymerase chain reaction
E) protein synthesis

F) A) and B)
G) B) and C)

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Which technique is preferred when investigators are trying to DNA fingerprint an individual?


A) short tandem repeat (STR)
B) polymerase chain reaction (PCR)
C) recombinant DNA (rDNA)
D) gene cloning
E) none of these

F) None of the above
G) B) and D)

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A

The purpose of PCR is to create


A) fragments of DNA that are different lengths.
B) recombinant DNA.
C) billions of copies of a segment of DNA.
D) plasmids to be used as vectors in cloning.
E) transgenic crop plants.

F) A) and E)
G) All of the above

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How can comparative genomics assist in the treatment of HIV?


A) The genome of HIV can now be compared to the human genome to find similarities between the two.
B) Knowing the genome of HIV allows for the manufacture of designer drugs to treat specific strains.
C) Understanding the genetic code of HIV allows scientists to replicate it in the laboratory.
D) Understanding the evolution of HIV in an individual will help scientists understand how the virus responds to different drug regimes and will lead to better treatments.
E) Knowing the proteins produced by HIV will allow the development of drugs to mimic those proteins.

F) C) and E)
G) A) and E)

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Which of the following procedures would most likely be used to identify a specific individual of a population?


A) DNA cloning
B) transgenic DNA
C) polymerase chain reaction
D) DNA fingerprinting
E) genetic engineering

F) A) and B)
G) C) and D)

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All of the uses listed below are uses for transgenic bacteria,except


A) Bioremediation, when bacteria are used to clean up spills of oil or other toxic substances.
B) The production of organic chemicals such as phenylalanine used in the production of aspartame.
C) The production of chemicals toxic to insects that can be used to protect plants from insects.
D) The production of plants like the pomato, it has a starchy root like the potato and also produces tomatoes on the stem portion of the plant.
E) The production of human growth hormones.

F) B) and C)
G) A) and B)

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List the number of chromosomes and estimated number of genes for each of the following organisms: 1.Homo sapiens 2.Mus musculus 3.Drosophilia melanogaster 4.Caenorhabditis elegans 5.Saccharomyces cerevisiae

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Species # of Chromosomes # of ...

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List three sites of ex vivo gene therapy and indicate the type of therapy being conducted.

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1.Skin: gene transfer by modifying blood...

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Which of the following statements was discovered by information gained from the human genome project?


A) Large sections of the human genome do not code for a polypeptide.
B) Nucleotides are composed of a pentose sugar, a phosphate group, and one of four nitrogenous bases.
C) DNA carries the information to make a polypeptide.
D) Regulation of gene expression determines the specialization of cells in humans.
E) DNA carries the information to make mRNA.

F) B) and D)
G) B) and E)

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Which of the following is NOT a concern over the use of genetically engineered corn?


A) resistance among populations of certain pests
B) resistance to herbicides
C) genetic exchange between engineered plants and related species
D) impact of engineered plants on nontarget plant species
E) presence of allergens

F) A) and C)
G) A) and E)

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The genome of Homo sapiens and Mus musculus are approximately the same size and composed of the same number of genes.

A) True
B) False

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Genetic markers show the presence of a predisposition to a disease or condition by


A) showing if the person produces the proteins associated with the disease or condition.
B) showing changes in the DNA that will cause the disease or condition.
C) stimulating the DNA to cause the symptoms of the disease or condition.
D) showing the specific number of DNA fragments associated with the disease or condition after cutting by restriction enzymes.
E) causing the affected area on the patient to fluoresce.

F) None of the above
G) A) and B)

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The molecule DNA ligase that is used to seal the piece of foreign DNA into the vector is


A) created for each specific plasmid.
B) created for each specific foreign DNA to be inserted.
C) naturally occurring in humans and is removed from humans and placed in the bacteria to create rDNA.
D) naturally occurring in both humans and bacteria and functions in DNA replication.
E) genetically engineered for each experiment.

F) A) and C)
G) C) and D)

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When human DNA is inserted into a bacterial plasmid the resulting bacterium will then


A) produce the products of the gene that has been inserted.
B) be placed into a vaccine and used to fight off viruses that attack humans.
C) be used to study the evolutionary relationship between humans and bacteria.
D) produce restriction enzymes that will help fight off viruses that attack humans.
E) be used to create DNA to insert the human genome.

F) A) and E)
G) B) and C)

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In DNA fingerprinting the products of PCR are cut by restriction enzymes into unique lengths; these fragments are then


A) Subjected to fluorescent dyes and visualized under an electron microscope.
B) Separated according to their relative positive and negative charges.
C) Separated according to the lengths of the fragments.
D) Arranged into a karyotype.
E) Used to produce the genes that give the individual a unique set of fingerprints.

F) C) and E)
G) B) and C)

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