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Transposable elements make up 45% of the human genome.What is thought to be their function?


A) They fight cancer.
B) They code for rRNA.
C) They determine gender.
D) They help in the replication of DNA.
E) We do not know their function.

F) B) and D)
G) A) and C)

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When creating transgenic bacteria,plants,and animals


A) any cell may be used as long as is does not have a cell wall.
B) only eggs may be used for animals while and cell may be used for plants and bacteria.
C) only eggs may be used for plants and animals while any cell may be used for bacteria.
D) only eggs may be used to create any transgenic organism.
E) transgenic bacteria are created first and then used to create transgenic plants and animals.

F) B) and E)
G) A) and D)

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The treatment of a disorder by inserting genetic material into an organism is called


A) karyotyping.
B) amniocentesis.
C) genetic profiling.
D) chorionic villi sampling.
E) gene therapy.

F) All of the above
G) A) and C)

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Bacteria,plants,and animals have all been used to create transgenic forms that are useful in


A) Producing high-yield tomato plants.
B) Creating pest resistant versions of corn.
C) Cleaning up oil spills.
D) Generating pharmaceuticals.
E) Producing new types of high yield beef.

F) A) and B)
G) None of the above

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One of the problems that limits the use of gene therapy is


A) the finding of suitable donors.
B) some patients are allergic to the bacterial DNA used.
C) mutation of the patient's genes caused by the therapy.
D) detrimental side effects from the inserted genes.
E) all of the above are problems with gene therapy.

F) A) and E)
G) D) and E)

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All of the following are benefits of sequencing the human genome except


A) knowing the exact sequence of DNA in an individual could allow pharmaceutical companies to design drugs for their exact genotype.
B) the discovery of the chemical makeup of DNA.
C) determining individuals who have a predisposing factor that could lead to future illness could allow for earlier treatment.
D) the improvement of sequencing technology that will allow the genomes of other species to be more readily sequenced.
E) enabling scientists to sequence the genome of various other organisms.

F) A) and C)
G) All of the above

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________ uses an electrical field to separate DNA fragments based upon their length.


A) DNA cloning
B) Polymerase chain reaction
C) Transgenics
D) Gel electrophoresis
E) DNA digestion

F) A) and B)
G) B) and E)

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Goats and cattle are used in gene pharming because


A) they are the easiest farm animals to clone.
B) therapeutic and diagnostic proteins are produced in the milk of these animals and can be harvested for use.
C) the diagnostic and therapeutic proteins produced can be passed to humans who consume their meat.
D) there are more of these two types of farm animals than other types.
E) it is easier to create recombinant DNA in these two farm animals than it is in other types.

F) C) and D)
G) A) and D)

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Why must an engineered DNA polymerase be used in PCR and not the one that occurs naturally?


A) It needs to work at very cold temperatures.
B) It needs to attach to DNA without a primer.
C) It must work in the 3' to 5' direction adding nucleotides to the 5' end.
D) It must work on single stranded DNA at high temperatures and attach to an oligonucleotide primer.
E) It must attach without a primer, work in the 3' to 5' direction and function at very cold temperatures.

F) A) and B)
G) A) and C)

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When creating transgenic organisms one major difference between plants and animals is that


A) only embryos can be used to create transgenic animals while any plant cell can be used for transgenic plants.
B) only eggs can be used to create transgenic animals while embryos can be used for transgenic plants.
C) only eggs can be used to create transgenic animals while any plant cell can be used for transgenic plants.
D) only embryos can be used to create transgenic animals while eggs must be used to create transgenic plants.
E) any cell may be used to create transgenic animals while embryos can be used for transgenic plants.

F) None of the above
G) C) and D)

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The human genome project discovered many small regions of DNA that vary among individuals called single nucleotide polymorphisms (SNPs) .Certain SNP patterns change an individual's susceptibility to disease and after response to medical treatments.This knowledge has lead to the idea


A) of designer drugs that are made to match one specific individual's DNA profile.
B) of designer babies that have known genetic traits.
C) that not everyone responds to drugs in the same way.
D) that not all humans are as well suited for survival.
E) that some DNA does not code for proteins.

F) C) and D)
G) B) and D)

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The human genome project lead to the discovery that much of the genome consists of repeated sequences of nucleotides.These are thought to


A) have no function.
B) function as protection on the ends of DNA and as an attachment site on the centromere.
C) function in DNA replication.
D) produce rRNA.
E) function as a protection against cancer.

F) All of the above
G) B) and E)

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What structure is used to seal the DNA into an opening created by the restriction enzyme during recombinant DNA technology?


A) DNA ligase
B) restriction enzymes
C) plamids
D) vectors
E) DNA helicase

F) B) and E)
G) A) and E)

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Which technique is used to create billions of copies of DNA in a short amount of time?


A) polymerase chain reaction
B) recombinant DNA technology
C) short tandem repeat (STR) profiling
D) gene therapy
E) none of the above

F) A) and B)
G) A) and C)

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Which animals have had the bovine growth hormone injected into them in order to produce larger individuals?


A) all of these
B) cows
C) fish
D) pigs
E) sheep

F) B) and C)
G) A) and D)

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________ is the application of computer technologies to the study of the genome.


A) Proteomics
B) Genomics
C) Bioinformatics
D) DNA fingerprinting
E) Genetic profiling

F) C) and E)
G) A) and B)

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Which of the following is not a use of DNA fingerprinting?


A) Paternity tests
B) Identifying Superbowl footballs
C) Forensic analysis
D) Determining a predisposition to cancer
E) All of the above are uses of DNA fingerprinting

F) A) and B)
G) C) and D)

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PCR is often used in forensic analysis since


A) it requires no specialized equipment.
B) untrained personnel can carry out the procedure.
C) very small samples are frequently all that are available.
D) juries always trust the method.
E) it has been proven to determine the criminal.

F) B) and E)
G) A) and D)

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The aim of functional genomics is to understand the function of the various genes discovered within each genomic sequence and how these genes interact.

A) True
B) False

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Which of the following is NOT used in the production of recombinant DNA (rDNA) ?


A) vectors
B) restriction enzymes
C) DNA ligase
D) RNA
E) plasmids

F) A) and D)
G) D) and E)

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