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In 1668, Francesco Redi conducted experiments to investigate the concept of spontaneous generation.He began by putting pieces of meat into identical jars.Some jars were left open to the air, some were sealed, and some were covered with gauze that kept out flies while allowing the meat to be exposed to the air.Redi then observed whether or not fly maggots appeared in the meat.What hypothesis was Redi testing?


A) Spontaneous generation is more likely to occur during the hot days of summer.
B) Maggots do not arise spontaneously, but from eggs laid by adult flies.
C) The type of meat affects whether spontaneous generation occurs.
D) Spontaneous generation can occur only if air is present.
E) Flies will develop from rotting meat if the meat is exposed to air.

F) A) and B)
G) C) and D)

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In 1972, Stanley Miller filled test tubes with ammonia gas, water vapor, and cyanide (HCN) , and he kept these tubes at 78°C for 27 years.What was found in these test tubes after 27 years?


A) Cyanobacteria
B) Amino acids and nucleotide bases
C) Pentose sugars
D) Pockets of oxygen
E) Protocells assembled from self-organizing fatty acids

F) A) and B)
G) A) and C)

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Whether the process is abiotic or occurs in an organism, the synthesis of macromolecules


A) occurs with an increase of entropy.
B) is spontaneous.
C) requires energy.
D) results in a more random system.
E) gives off heat.

F) D) and E)
G) A) and E)

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After the discovery that volcanoes released carbon dioxide (CO2) , hydrogen sulfide (H2S) , and sulfur dioxide (SO2) into the atmosphere 4 billion years ago, scientists conducted the Miller‒Urey experiment with the inclusion of these gases.How did the results differ from the original results?


A) No small or large prebiotic molecules were produced.
B) DNA was produced.
C) All 20 amino acids used in protein synthesis were produced.
D) Living cells were produced.
E) Nonliving protocells were produced.

F) A) and C)
G) C) and D)

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Meteorites from space


A) carried cyanobacteria that provided Earth's first oxygen.
B) carried retroviruses from which DNA molecules have been synthesized in the lab.
C) show no chemical evidence of water on Mars.
D) contain purines, pyrimidines, sugars, and ten amino acids.
E) carried amino acids that were all l-amino acids, the predominant form found in Earth organisms.

F) A) and D)
G) A) and C)

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Which characteristic of living cells is also a characteristic of protocells?


A) The capacity for reproduction by division
B) Replication of nucleic acids using external nucleotides
C) Recognition of other cells
D) The capacity for carrying out metabolic reactions
E) Transfer of biological information to new cells

F) A) and B)
G) B) and D)

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Unlike RNA, DNA contains


A) cytosine.
B) uracil.
C) thymine.
D) guanine.
E) adenine.

F) B) and E)
G) B) and D)

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Refer to the figure below showing a protocell. Refer to the figure below showing a protocell.   Identify the process depicted inside the protocell, and explain why its rate would be enhanced inside the protocell compared with its rate outside the protocell. Identify the process depicted inside the protocell, and explain why its rate would be enhanced inside the protocell compared with its rate outside the protocell.

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The protocell contains a group of nucleo...

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Because it is easy to spot meteorites against white snow and ice, Antarctica is a place where teams of scientists routinely go to search for them.When a meteorite found in Antarctica is analyzed and found to contain amino acids, scientists should exercise caution before claiming that the molecules are evidence that life came to Earth from other planets.Which statement best applies in this situation?


A) The amino acids must be an equal mixture of d- and l-isomers to be considered evidence of life.
B) The molecules should include all 20 standard amino acids found in living organisms on Earth.
C) Organic molecules on the meteorite may be from Earth organisms that contaminated it after it fell to Earth.
D) The extreme cold of Antarctica could have favored unusual chemical reactions in the meteorite before it was picked up.
E) Exposure to the sun as the meteorite lay on the snow and ice could increase the likelihood that organic molecules would form on it.

F) B) and C)
G) C) and D)

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What is the sequence of the complementary strand of DNA given the template DNA sequence below? (Note: Spaces between nucleotides have no purpose but to make the sequences easier to read.) What is the sequence of the complementary strand of DNA given the template DNA sequence below? (Note: Spaces between nucleotides have no purpose but to make the sequences easier to read.)    A)  5<font face= symbol ></font>-GCC TCG GAA CGT CTT CGA-3<font face= symbol ></font> B)  3<font face= symbol ></font>-GCC CTG GAA CGT CTT CGA-5<font face= symbol ></font> C)  3<font face= symbol ></font>-GCC UCG GAA CGT CUU CGA-5<font face= symbol ></font> D)  5<font face= symbol ></font>-CGA GGC GTC AAC TCG CTT-3<font face= symbol ></font> E)  3<font face= symbol ></font>-CGG AGC CTT GCT GAA GCT-5<font face= symbol ></font>


A) 5-GCC TCG GAA CGT CTT CGA-3
B) 3-GCC CTG GAA CGT CTT CGA-5
C) 3-GCC UCG GAA CGT CUU CGA-5
D) 5-CGA GGC GTC AAC TCG CTT-3
E) 3-CGG AGC CTT GCT GAA GCT-5

F) All of the above
G) B) and C)

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Refer to the table below, showing a portion of the DNA sequence of the same gene present in five different species. Refer to the table below, showing a portion of the DNA sequence of the same gene present in five different species.   Of the pairs listed, which two species are likely to be the most distantly related through evolution? A)  1 and 4 B)  2 and 3 C)  2 and 5 D)  3 and 5 E)  4 and 5 Of the pairs listed, which two species are likely to be the most distantly related through evolution?


A) 1 and 4
B) 2 and 3
C) 2 and 5
D) 3 and 5
E) 4 and 5

F) B) and D)
G) C) and D)

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The Miller-Urey experiments showed that biological molecules could be made from inorganic precursors.These results


A) support the theory that life's molecules originated outside Earth.
B) showed that RNA can catalyze reactions.
C) showed that only the l-isomers of amino acids were formed by living organisms.
D) support the chemical evolution theory.
E) showed how biopolymers are made.

F) None of the above
G) A) and B)

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What is the evidence supporting the claim that ancient rocks contain the fossils of cells?


A) The imprints of chloroplasts can be seen in the rocks.
B) Radioactive tracing of CO2 in the rocks shows similarities to modern cells.
C) Microscopic examination of the rocks shows chains of cells that are similar to modern cyanobacteria.
D) DNA analysis of the rocks shows similarities to modern cells.
E) Pieces of RNA found in the rocks are similar to ribozymes.

F) A) and C)
G) B) and E)

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In 1969, Australian scientists analyzed the structure of an uncontaminated portion of a meteorite.They found a number of the molecules that are necessary for life, including purines, pyrimidines, sugars, and ten amino acids.This result supports which statement?


A) The small molecules of life occur elsewhere than on Earth.
B) Life on Earth must have originated from extraterrestrial molecules.
C) The question of how life originated on Earth has been answered.
D) Other planets must support life.
E) Life arose in multiple places in the universe.

F) B) and E)
G) B) and C)

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Which statement concerning replication, transcription, and translation is correct?


A) Translation uses amino acids as building blocks.
B) Both transcription and translation use nucleotides as building blocks.
C) Both replication and transcription use amino acids as building blocks.
D) Replication uses amino acids as building blocks.
E) Both replication and translation use nucleotides as building blocks.

F) B) and E)
G) A) and D)

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Protocells are


A) organisms like viruses, with a protein coat.
B) the earliest known cells.
C) also known as cyanobacteria.
D) complexes formed in the lab by self-organizing fatty acids.
E) complexes formed from a ribozyme and a substrate.

F) A) and C)
G) C) and D)

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What properties of DNA structure make it well suited for its function as an informational molecule?

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DNA structure is a linear chain of regul...

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Nucleic acids and proteins are both polymers made of a set of monomers.Complete the following analogy: _______ are to nucleotides as side chains are to amino acids.


A) ribose/deoxyribose sugars
B) phosphate ions
C) nitrogenous bases
D) nucleosides
E) hydroxyl groups

F) C) and E)
G) A) and B)

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Refer to the figure below showing two strands of DNA. Refer to the figure below showing two strands of DNA.   Which statement about uracil can you infer to be true? A)  It has a double ring and forms two hydrogen bonds. B)  It has a single ring and forms two hydrogen bonds. C)  It has a double ring and forms three hydrogen bonds. D)  It has a single ring and forms three hydrogen bonds. E)  It has a single ring and forms one hydrogen bond. Which statement about uracil can you infer to be true?


A) It has a double ring and forms two hydrogen bonds.
B) It has a single ring and forms two hydrogen bonds.
C) It has a double ring and forms three hydrogen bonds.
D) It has a single ring and forms three hydrogen bonds.
E) It has a single ring and forms one hydrogen bond.

F) D) and E)
G) C) and E)

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Hexokinase is an enzyme that catalyzes the phosphorylation of glucose to produce glucose-1-phosphate.The reaction uses ATP as the source of phosphate, and ADP is the other product of the reaction.Each glucose-1-phosphate produced undergoes a series of reactions that lead to the formation of two pyruvate molecules, which contain three carbon atoms each.This is an example of a nucleotide functioning in


A) transfer of biological information.
B) breakdown of carbohydrates.
C) transport of cellular energy.
D) storage of cellular energy.
E) transfer of environmental information to cells.

F) B) and D)
G) B) and C)

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